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Page 68
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Cell Science, Stem Cell Research &
Pharmacological Regenerative Medicine
November 29-30, 2017 | Atlanta, USA
Annual Congress on
Adv cel sci tissue cul 2017 | Volume 1 Issue 2
In crustaceans, calcium signaling plays important roles in
growth, reproduction and molting. Calreticulin (CRT) is a
main protein involved in calcium homeostasis of eukaryotes.
The full-length cDNA of CRT in the giant tiger shrimp
(Penaeus monodon), identified by RACE-PCR, was 1682 bp
in length, containing an ORF of 1221 bp corresponding to
a deduced protein of 406 amino acids. Genomic sequence
of PmCRT spanned 3006 bp, composing of 4 exons (85,
119, 187 and 830 bp) and 3 introns (411, 231 and 120 bp).
Semi-quantitative RTPCR revealed that PmCRT in hemocytes
of juvenile P. monodon was up-regulated at 0 and 1 h post
treatment (hpt) at 35°C for 3 h (Pb0.05). However, expression
levels of PmCRT in gills and hepatopancreas after the
temperature stress (0-48 hpt) were not significantly different
(n=3 for each group; PN0.05). Quantitative real-time PCR
confirmed the expression profile of PmCRT in hemocytes
and illustrated that this transcript was up-regulated at 0 and
3 hpt for approximately 25 folds (n=5; Pb0.05), reduced to
about 5 folds between 3 and 12 hpt (Pb0.05) and returned
to the baseline level at 24 and 48 hpt (PN0.05). Recombinant
PmCRT was successfully expressed in vitro and exhibited an
ability to form a complex with recombinant endoplasmic
reticulumprotein 57 of P. monodon (rPmERp57). Results from
this study strongly suggested that PmCRT can be regarded as
a biomarker for temperature stress responses in P. monodon.
e:
Molecular characterization of calreticulin: A biomarker for temperature stress responses of the giant tiger
shrimp Penaeus monodon
Virak Visudtiphole1, Apiruck Watthanasurorot2, Sirawut Klinbunga1, 2, Piamsak Menasveta2 and Kanyawim Kirtikara1
1BIOTEC, Thailand
2Chulalongkorn University, Thailand