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academies
Cell Science, Stem Cell Research &
Pharmacological Regenerative Medicine
November 29-30, 2017 | Atlanta, USA
Annual Congress on
Adv cel sci tissue cul 2017 | Volume 1 Issue 2
D
ental pulp tissue has the potential to regenerate dentin
in response to stimulations. Thus, stem cell therapy
has considerable a promise in pulp dentin regeneration.
The aim of this study is an in-vivo evaluation of pulp’s stem
cells capacity in pulp and dentin regeneration in dogs. To
isolate stem cells, one Iranian mixed-breed, 5-months dog
was used. The deciduous tooth was extracted. Pulp of
the tooth was isolated and exposed to type 1 collagenase
enzyme. Isolated cells were cultured on Dulbecco’s modified
Eagle’s medium (DMEM), supplemented with 10% fetal
bovine serum (FBS) and 1% antibiotic. Polyglycolate (PGA)
scaffolds were prepared and sanitized in 75% ethanol and
seeded with 4×104 cells. Twenty anterior and premolar
dogs’ teeth underwent shallow pulpotomy. Then all teeth
were divided to three groups. Twelve teeth transplanted
with seeded scaffolds and then cavities were filled with
MTA and Amalgam. Control groups consisted of four teeth
with unseeded PGA restored with MTA and Amalgam and
four teeth with only MTA and Amalgam. Eight weeks after
transplantation, samples were histologically analyzed. Mann
Whitney U test was used to compare inflammation, calcific
barrier and hyperemia and Chi-square test to compare
necrosis and Odontoblastic layer formation. There was no
significant difference between 3 groups except for calcified
barrier type between group 1 and 2, Dentin like matrix,
collagen fibers and small vessels observed in the cavity in
group using stem cells. The results of the study suggest the
possibility of pulp and dentin regeneration with stem cells in
damaged teeth.
e:
Shirin_782000@yahoo.comStem cells from exfoliated deciduous teeth, a way for pulp and dentin regeneration (an animal study)
Shirin Shahnaseri
and
Seyed Amir Mousavi
Isfahan University of Medical Sciences, Iran