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allied
academies
Cell Science, Stem Cell Research &
Pharmacological Regenerative Medicine
November 29-30, 2017 | Atlanta, USA
Annual Congress on
Adv cel sci tissue cul 2017 | Volume 1 Issue 2
D
ocosahexaenoic acid (DHA) is an n-3 long chain
polyunsaturated fatty acid (PUFA) that is involved in a
wide range of cellular processes in human cells. Brain lipid
binding protein (BLBP) exhibits a high affinity for n-3 PUFAs,
especially DHA, but the precise functional contributions of
DHA and BLBP in astrocytes are not clear. We analyzed cell
viability and the ratio of Ki67 positive cells after manipulating
DHA and/or BLBP levels in cultured astrocytes and found that
10 μM (low-dose) DHA stimulated proliferation of astrocytes,
whereas this proliferative effect could be attenuated by
downregulation of BLBP. Moreover, we found that astrocyte
proliferation was directly regulated by BLBP independently
of DHA. Taken together, low-dose DHA-induced astrocyte
proliferation was disturbed by insufficient BLBP; and besides
acting as a fatty acid transporter, BLBP was also involved in
the proliferation of astrocytes directly.
e:
qinjb@ntu.edu.cnLow-dose DHA-induced astrocyte proliferation can be attenuated by insufficient expression of BLBP
in
vitro
Jianbing Qin, Guohua Jin
and
Haoming Li
Nantong University, China