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Cell Science, Stem Cell Research &
Pharmacological Regenerative Medicine
November 29-30, 2017 | Atlanta, USA
Annual Congress on
Adv cel sci tissue cul 2017 | Volume 1 Issue 2
Objective: The objective of the study is to investigate the
differentiation of bone marrow mesenchymal stem cells
(MSCs) into vascular endothelial cells in vitro.
Methods: MSCswere isolated frombonemarrowby attaching
growth and identified by flow cytometry. MSCs were seeded
in inducing medium supplemented with VEGF, bFGF, EGF and
IGF to differentiate into endothelial cells. The differentiated
cells were identified by measuring surface marks (CD31 and
CD34) on the 2nd, 6th, 10 th and 14 th day. qPCR was performed
to detect the expression of vWF and Flk-1 on mRNA level.
The functional behavior of the endothelial cells from MSCs
was tested by tube formation assay in vitro on matrigel gel.
Results: The primary MSCs demonstrated spindle-shaped
morphology and expressed stem cells marker. After two days
induction, CD31 and CD34 began to express in 7.27% cells.
The expression of CD31 and CD34 increased gradually during
inducing period and reached a peak on the fourteenth
day with 57.6% positive cells. qPCR demonstrated that
the expression of vWF and Flk-1 was significantly higher in
induced MSCs. The induced MSCs could form vessel-like
structures on matrigel gel.
Conclusion: MSCs can differentiate into functional
endothelial cells after induced by VEGF, bFGF, EGF and IGF
and form vessel-like structures in vitro , suggesting that
induced MSCs will be ideal seed cells for the treatment of
lower extremity atherosclerotic occlusive disease.
e:
Differentiation of human messenchymal stem cells into vascular endothelial cells
Chengen Wang, Min Yang, Yuan Li, Huihui Liu, Zeyin Liang, Yue Yin, Xiaoqiang Tong, Li Song, Guochen Niu, Ziguang Yan, Bihui Zhang and Yinghua Zou
Peking University First Hospital, China