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Page 70
allied
academies
Cell Science, Stem Cell Research &
Pharmacological Regenerative Medicine
November 29-30, 2017 | Atlanta, USA
Annual Congress on
Adv cel sci tissue cul 2017 | Volume 1 Issue 2
S
tress of cultured species is a common problem, affecting
the host health, in aquatic farming. It occurs when
interactions among the three elements – host, environment
andpathogens,arenot inbalance, particularlyunder intensive
culturing conditions. Hosts respond to stress by triggering
cellular Ca2+ signaling to activate many downstream
pathways for their functions in order to mitigate the stress
effect. Calreticulin (CRT), Calnexin (CNX) and Endoplasmic
reticulum protein 57 (ERp57) are involved in Ca2+ signaling
in stress response mechanisms and are essential for survival
and viability of organisms under stress conditions. In this
study, the full length cDNA of CNX and ERp57 in the giant
tiger shrimp (
Penaeus monodon
) were identified. Amino acid
sequences deduced from both cDNA showed significantly
high similarity with those present in other crustaceans. One
and two forms of PmCNX and PmERp57 transcripts were
found, respectively. For PmERp57, its genomic sequence
was additionally identified. It spanned 8257 base pairs (bp),
composing of10 exons (84–226 bp long) and 9 introns (93–
2787 bp long). Both genes were expressed in all examined
tissues of both juvenile and broodstock
P. monodon
. Effects
of Vibrio harveyi and abotic stress commonly found in
aquaculture on expression of PmCRT, PmCNX and PmERp57
were examined in hemocytes by quantitative real time
PCR. High temperature, hypo- and hyper-osmosis and
V. harveyi
infection induced expression all of the three
genes (P<0.05). In contrast, analysis of hypoxia effect on
expression of the Ca2+ signaling-related genes, which was
firstly studied in aquatic species, showed to suppress their
expression (P<0.05). Results from this study suggested that
PmCRT, PmCNX and PmERp57 are related to stress response
mechanisms and immune system of
P. monodon
. Their
expression is therefore a potential candidate for markers
to detect or monitor the early sign of shrimp’s stress and
immunity changes in farming.
e:
Virak.Vis@biotec.or.thCalcium signaling-related genes in
Penaeus monodon
respond to abiotic stress and pathogenic bacteria
common in aquaculture
Virak Vasthudipole
National Science and Technology Development Agency- BIOTEC, Thailand