cell-science-pharmacology-2017-posters-abstracts

15 / 28

Page 58
allied
academies
Cell Science, Stem Cell Research &
Pharmacological Regenerative Medicine
November 29-30, 2017 | Atlanta, USA
Annual Congress on
Adv cel sci tissue cul 2017 | Volume 1 Issue 2
The aim of the study is to explore the function of
brain lipid binding protein in the proliferation of rats’
astrocytes in vitro. The BLBP overexpressed adenovirus or
small interference RNA (siRNA) was used to transfect into
astrocytes. 3 days later, the gene and protein expression of
BLBP was detected, the proportion of Ki67 and EdU positive
cells was measured and the cell cyclewas investigated by flow
cytometry methods. After treatment of BLBP adenovirus,
the expression of BLBP gene was elevated, the exogenous
expression of BLBP could be expressed in astrocytes, the
proportion of Ki67 and EdU positive cells was also increased,
as well as the proportion of cells at S phase. After treatment
of BLBP siRNA, the expression of BLBP gene was decreased,
the proportion of Ki67 and the cells at S phase was reduced
as well. In conclusion, besides as a fatty acids transporter,
BLBP also regulates the proliferation of astrocytes in vitro.
e:
Brain lipid binding protein (BLBP) regulates the proliferation of astrocytes in vitro
Haoming Li, Jianbing Qin and Guohua Jin
Medical School of Nantong University, China