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World Biochem 2019 & Regenerative Medicine 2019
Journal of Genetics and Molecular Biology | Volume 3
Page 36
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
alliedacademies.comYEARS
March 25-26, 2019 | Amsterdam, Netherlands
&
BIOCHEMISTRY AND ENZYMOLOGY
World Congress on
TISSUE ENGINEERING AND REGENERATIVE MEDICINE, STEM CELL RESEARCH
2
nd
Global Conference on
Joint Event on
SITE-DEPENDENT VARIATION OF LINEAGE
PREFERENCE FROM ADIPOSE STEM CELLS
S
tem cells from subcutaneous adipose tissue (ScASCs) are considered a potential
cell source for cartilage regeneration. Unfortunately, the capacity of ScASCs toward
chondrogenesis is very limited. Interestingly, recent reports indicate that stem cells
from infrapatellar fat pad (IPFSCs), an adipose tissue depot next to knee joints, exhibit
an excellent potential to differentiate into cartilage. Despite the fact that some studies
have investigated these two kinds of adipose stem cells in chondrogenic differentia-
tion, few reports are available to compare adult stemcells from these two tissues in ge-
nome sequencing and protein composition. ScASCs and donor-matched IPFSCs were
isolated from four 4-month-old healthy rabbits. Our cell proliferation assay showed
that ScASCs proliferated faster than IPFSCs, while IPFSCs exhibited much higher ex-
pression of CD146, a marker related to pericytes, stemness, and maybe chondrogen-
esis. IPFSCs exhibited obvious preference for chondrogenic differentiation based on
the expression of
SOX9, COL1A1, COL2A1
, and ACAN (p<0.05), while ScASCs were pref-
erential for adipogenic differentiation based on the expression of
ADIPOQ, LPL, PPARG
,
and
LEP
, and the synthesis of adiponection (p<0.05). None of them showed priority in
osteogenesis based on the expression of BGLAP, RUNX2, DCN, and SPARC, and the syn-
thesis of osteocalcin (p>0.05). Expression of stemness and senescence related genes
(
NANOG, REX1, NES, SOX2, CDKNIA, TP53
) indicated that no differences were evident
between both groups (p>0.05). Proteomes of IPFSCs and ScASCs are uniquely dis-
tinguishable for both the cells and the secreted ECM using both partial least squares
discriminant analysis (PLS-DA) and volcano plot. The finding is also consistent with
the data from a principal component analysis (PCA) of RNA sequencing. The results
demonstrated that, despite similar source from adipose tissue, the preference for
differentiation lineage is depot dependent, indicating that local microenvironment
might dominate the fate of local stem cells. Our findings suggested that IPFSCs are
more suitable for cartilage regeneration while ScASCs tend to differentiate toward
adipose tissue.
Ming Pei, J Genet Mol Biol 2019, Volume 3
Ming Pei completed his PhD from Beijing
University, China and postdoc training from
Harvard-MIT Division of health sciences and
Technology, USA. Currently he is a tenured
professor and director of stem cell and tissue
engineering laboratory in the department of
orthopaedics, West Virginia University, USA.
He has over 100 publications that have been
cited over 3100 times, and his publication
H-index is 32 and has been serving as an edi-
torial board member of reputed Journals.
mpei@hsc.wvu.eduMing Pei
West Virginia University, USA
BIOGRAPHY