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World Biochem 2019 & Regenerative Medicine 2019
Journal of Genetics and Molecular Biology | Volume 3
Page 39
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
alliedacademies.comYEARS
March 25-26, 2019 | Amsterdam, Netherlands
&
BIOCHEMISTRY AND ENZYMOLOGY
World Congress on
TISSUE ENGINEERING AND REGENERATIVE MEDICINE, STEM CELL RESEARCH
2
nd
Global Conference on
Joint Event on
CURCUMIN BASED FLUORESCENT
CHEMOSENSOR FOR SELECTIVE
DETECTION OF CU
2+
AND HG
2+
IONS
IN AQUEOUS MEDIUM AND CELL
IMAGING STUDY
C
urcumin based fluorescent Schiff base chemosensor L2 (4,4’-((1E,3Z,5E,6E)
3-hydroxy- 5-(thiazol-2-ylimino) hepta1,3,6-triene-1,7-diyl) bis(2-methoxy
phenol) shows notable change in absorbance and emission spectra on screen-
ing with some metal ions (Fe
3+
, Cu
2+
, Ni
2+
, Zn
2+
, Cd
2+
, Hg
2+
, Pb
2+
, Cr
3+
, Mn
2+
, Co
2+
,
Ag
1+
.etc.). in HEPES buffer. Bnding constant for L2 + Cu
2+
(2.4 x 10
5
M
-1
) and L2
+ Hg2+ (7.46 x 10
5
M
-1
) which indicate good interaction of chemosensor with
Cu
2+
and Hg
2+
ions. An interesting value of detection limit was observed with
Cu
2+
and Hg
2+
ions, was to be 2.0 and 1.2µM, respectively, which further clear
the selectivity of the molecular probe towards Cu
2+
and Hg
2+
ions respectively.
Detection of Cu
2+
ions by chemosensor L2 in to biological cells was performed
by using fluorescence cell-imaging study. Staining cells only with chemosen-
sor exhibited intercellular fluorescence and upon the addition of Cu
2+
ions the
cells lost their fluorescence. The cell imaging studies clearly indicate that che-
mosensor L2 has good cell permeability and show the formation of L2– Cu
2+
complex.
Sunita Hooda, J Genet Mol Biol 2019, Volume 3
Sunita Hooda obtained her PhD degree in
physical chemistry in 1992 from Indian Insti-
tute of Technology Delhi, India. She is pres-
ently, working as an associate professor in
the Department of Chemistry, Acharya Nar-
endra Dev College (University of Delhi). She
has expertise in NMR studies of synthesized
polymers. She publishes 53 research papers
in reputed national and international jour-
nals. Her current research area of interest is
“Synthesis of Polymeric and Heterocyclic
Chemosensors for Cations, Anions and Mol-
ecules Recognition”.
sunitahooda@rediffmail.comSunita Hooda
University of Delhi, India
BIOGRAPHY