world-biochem-regenerative-medicine-2019-keynote

4 / 11

World Biochem 2019 & Regenerative Medicine 2019 Journal of Genetics and Molecular Biology | Volume 3
Page 24
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
March 25-26, 2019 | Amsterdam, Netherlands
&
BIOCHEMISTRY AND ENZYMOLOGY
World Congress on
TISSUE ENGINEERING AND REGENERATIVE MEDICINE, STEM CELL RESEARCH
2nd Global Conference on
Joint Event on
DEVELOPING OF TRILOBATIN DIHYDROCHALCONE
AS A NEW-STYLE OF PHYTOCHEMICAL AGENT
The increasing prevalence of several diseases, like Alzheimer’s disease,
obesity, cancer, and diabetes, in humans in recent decades worldwide,
accompanied by rising concern regarding the safety of many synthetic
chemistry-based pharmaceuticals, has raised public demand for phyto-
chemical-based medicines. This in turn has led to increasing interest in
metabolic engineering as an approach to produce such natural products
on an industrial scale, which has the potential to decrease production
costs of, eg desired dihydrochalcones.
Wenote that fruits accumulatinghigh level of phytochemicals includingfla-
vonoids and dihydrochalcones that may play a key role in reducing chronic
disease risk. We have developed a novel concept to produce a new-style
phytochemical agent of benefit for humans by genetic transformation of
three characterized genes in plant cells, bacterial, and yeast systems. We
have applied a set of molecular and biochemical tools to identified reac-
tions and enzymes leading to the biosynthesis of dihydrochalcones. We
recently cloned and biochemically characterized three key enzymes in the
dihydrochalcone biosynthesis pathway; a p-coumaroyl-CoA double bond
reductase that converts p-coumaroyl-CoA into p-dihydrocoumaroyl-CoA,
chalcone synthase that accepted p-dihydrocoumaroyl-CoA, in the pres-
ence of malonyl-CoA, leading to production of phloretin, and a specialized
phloretin-4’-O-glycosyltransferse which glycosylated phloretin in the pres-
ence of UDP-glucose into trilobatin. The production of the phytochemical
trilobatin was achived by overexpression of these genes in microbial cell
factory. The proof of our concept have open the possibility for developing
new-style of natural phytochemical for a growing market of population
suffering from“modern”diseases.
Mwafaq Ibdah, J Genet Mol Biol 2019, Volume 3
Mwafaq Ibdah has completed his PhD at the
age of 32 years from Martin Luther University,
Hale/Salle, Germany and postdoctoral studies
fromMichigan State Universtity andWashing-
ton State University. He is tenured researcher
at NeweYa’ar Research Center, The Agriculture
Research Organization (ARO), Isarel. He has
published more than 20 papers in reputed
journals and has been serving as an editorial
board member of repute.
Mwafaq Ibdah
Agriculture Research Organization, Israel
BIOGRAPHY