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Journal of Biomedical Research | ISSN: 0976-1683 | Volume 30
March 14-15, 2019 | London, UK
T issue Engineer ing, Stem Cel ls and Regenerat ive Medicine
Cel l and Gene Therapy
World Congress on
International Conference on
&
Joint Event
The role of dissolved oxygen level on human mesenchymal stem cells culture and its implication on
the cryopreservation process
Soukaina Bahsoun, K Coopman
and
E Akam
Loughborough University, UK
H
uman mesenchymal stem cells (hMSCs) are viewed by
many as strong candidates for cell therapy. The translation
from bench to bed side requires an expansion process with no
compromise on the cells’ safety, viability, purity and potency.
hMSCs culture conditions have been the subject of vast
research and a debate still exists on how to describe hMSCs
“best performance”. Culturing hMSCs in low oxygen (hypoxia)
has become a popular option aiming to improve yield and
functionality. A review of the literature was conducted by
(Bahsoun et al. 2018) to gather evidence on howhypoxia affects
hMSCs attributes including marker expression, differentiation
potential, growth, attachment, migration, genomic stability
and paracrine activity. Despite the disparities noticed across
the literature in the terminology and the equipment used, it
was concluded that hypoxia improves most of the attributes
assessed.
Cryopreserved human bone marrow mesenchymal stem
cells (hBM-MSCs) are one of the most common types
of cells used in clinical trials. Whether autologous or
allogeneic, cryopreservation is an integral part of cell therapy
manufacturing. While using cryopreserved cells is sometimes
taken for granted, developing optimal cryopreservation
processes is still a challenge. Using hypoxia pre-conditioning to
improve hBM-MSCs recovery after cryopreservation is a novel
approach. Preliminary data shows hypoxia pre-conditioning
improves the post-thaw osteogenic potential of hBM-MSCs.
Speaker Biography
Soukaina Bahsoun has completed a three-year degree in biology. She moved to the
University of Victoria in Canada and studied five high-level molecular biology modules, two
of which are directed studies completed under the supervision of professor David Levin and
professor Francis Choy. After moving to UK, she joined the Open University and completed
Bachelor of Science (with honours) first class degree. Her motivation for graduate studies
and research allowed her to secure a position on the postragduate training programme in
regenerative medicine at Loughborough University, UK.
e:
S.bahsoun@lboro.ac.ukSoukaina Bahsoun et al.
, Biomed Res, Volume 30
DOI: 10.4066/biomedicalresearch-C1-026