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Biomedical Research
|
Volume 29
Page 40
Note:
allied
academies
CARDIOLOGY AND CARDIOVASCULAR MEDICINE
STEM CELLS AND REGENERATIVE MEDICINE
&
International Conference on
International Conference on
J u n e 1 8 - 1 9 , 2 0 1 8 | O s a k a , J a p a n
Joint Event on
EFFECTS OF LOW DOSE RADIATION
ON DEVELOPING HUMAN RETINAL
GANGLION CELLS FROM INDUCED
PLURIPOTENT STEM CELLS
Mari Katsura
University of Tokyo, Japan
R
etinal ganglion cells (RGCs) are essential components for vision,
whose long axons link to the visual field in the brain. Loss of RGC is
often in the eyes observable in the eyes with various visual disorders, such
as glaucoma and ischemia. Developments of RGCs start at gestational
5 weeks earlier than other kinds of neural cells in the retina. However,
regeneration of RGC has never been reported in mammalian eyes so far.
Instead, small amount of loss in RGC does not cause visual disturbance
which may be supported by some surplus of RGCs in retina. To validate
the effects of low dose radiation on the differentiation of RGC, we have
established a protocol to differentiate iPS into RGCs within 35 days. We
applied low dose irradiation of 30 mGy and 180 mGy for 24 hours from
day 4 to day 5 and observed that the axagonal elongation was interfered.
To dissect molecular mechanism of this finding, we performed a series of
transcriptome analysis and extracted a group of genes, including PAX6,
which were down regulated in a dose dependent manner. To identify
radiation dependent gene regulation, we performed epigenetic analysis
to identify active enhancers in affected genes. Based on H3K4me3 and
H3K27ac localization, we work on motif analysis to identify consensus
transcription factor binding sites could be observed. Currently, we are
recollecting time course data and trying to find the earliest responsive
transcription factor in low dose irradiation stimulation.
Mari Katsura is graduated from Hiroshima Uni-
versity Medical School, Hiroshima, Japan. She
has been an ophthalmologist after graduation.
Now her work is research for the effects of low
dose radiation on human health, especially in
neural cells. Her laboratory is in Isotope science
center, The University of Tokyo, where many re-
searchers from different fields are working co-
operatively.
marikatsura-tky@umin.ac.jpBIOGRAPHY
Mari Katsura, Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C2-006