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Journal of Agricultural Science and Botany | Volume: 2
November 15-16, 2018 | Paris, France
Plant Science
Natural Products,Medicinal Plants and Traditional Medicines
International Conference on
Joint Event
&
Fluorescence based kompetitive (competitive) allele specific PCR (KASP) for high-throughput SNP
marker detection and validation
Tabassum Rahman Sunfi
University of Dhaka, Bangladesh
Considering the enormous potential of DNA markers in
plant breeding and recent advances in single nucleotide
polymorphism (SNP) genotyping for its promising role in crop
improvement, it is crucial for plant breeders to adopt the
capacity of SNP marker development and marker assisted
selection. However, cost of utilizing high throughput SNP
detection system is possibly the most important factor that
limits the implementation ofmarker assisted selection (MAS). In
the current work, we have attempted to establish fluorescence-
based Kompetitive Allele Specific PCR (KASP) technology for
easy and efficient detection of SNP alleles and validation of
SNP based quantitative trait loci (QTL). A mapping population
at F6 and F7 with the salt tolerant rice landrace Horkuch and
sensitive but high yielding IR29, was used to establish KASP
genotyping. Specific salt tolerance SNP-based QTLs had been
previously identified at the F2-3 stage from this mapping
population, with IR29 ( ) and Horkuch ( ). KASP markers were
designed and genotyping assay was done with F6 sample DNA
where polymorphism in seven out of eight SNP markers were
detected. Based on physiological analysis for the presence of
desired QTLs, a subset of plants were chosen and advanced
to F7 generation and SNP based QTLs were validated applying
KASP assay. Hence, in the overall study KASP genotyping
method was found more suitable as a marker validation system
than other methods due to its high accuracy, low cost, flexibility
in assay design and fluorescence based detection method.
Therefore, was used further for potential donor (tolerant) plants
selection that can be used inmarker assisted breeding program.
e:
Plant science & Natural Medicine 2018, Volume 2
DOI: 10.4066/2591-7897-C1-003