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alliedacademies.comYEARS
Pharma Summit 2018 & Gastro Summit 2018 Asian Journal of Biomedical and Pharmaceutical Sciences | ISSN: 2249-622X | Volume 8
&
GLOBAL PHARMA SUMMIT
GASTROENTEROLOGY AND HEPATOLOGY
2
nd
International Conference on
Praveen Srikumar Polu, Asian J Biomed Pharmaceut Sci 2018, Volume 8 | DOI: 10.4066/2249-622X-C5-014
PHYTOCHEMICAL INVESTIGATION AND
IN VITRO
PRELIMINARY ANTIMITOTIC
STUDIES ON ETHANOLIC EXTRACT (ETOH)
OF AERIAL PARTS OF
LEUCAS DIFFUSA
.
BENTH
Praveen Srikumar Polu
MRM College of Pharmacy, India
L
ucas Diffusa
commonly known as Thumbai (Tamil) decumbent herb is
widely distributed in Asian countries and some places in India. The objec-
tive of present study was to investigate the presence of various phytochem-
ical constituents and preliminary antimitotic screening for ethanolic extract.
The crude EtOH extract of aerial plant of
Leucas diffusa
were taken under
phytochemical investigation revealed the presence of steroids, triterpenoids,
flavanoids and carbohydrates. The extract was fractioned and isolated from
n-Hexane, Ethylacetate, and Chloroform partitionates by using column chro-
matography and preparative TLC techniques. The IR spectra of the EtOH
extract indicated the presence of OH, C=O, C-H, C-O-C and NH. Cytotoxic
properties of plant extracts being developed for cancer treatment are usually
evaluated by a variety of
in vivo
and
in vitro
tests carried out in animal or plant
based models. In the present study we have evaluated the possibility of using
plant model the germinating Mung beans (
Vigna radiata
), for rapid and inex-
pensive screening of extract exhibiting cytotoxic properties. The EtOH extract
showed strong inhibitory effect on seed germination at 100µg/mL, 250µg/
mL and 500µg/mL.
Our study shows that germinating
V. radiata
beans could be used as a conve-
nient model for the preliminary screening of drugs exhibiting cytotoxic prop-
erties.
Praveen Srikumar Polu Working as Professor in the De-
partment of Pharmaceutical Analysis in MRM College
of Pharmacy, Hyderabad. He has done the PhD Doctor
of Philosophy in 2010 Awarded from Andhra University,
Visakhapatnam, in Faculty of Pharmaceutical Scienc-
es. Professional Diploma In Recent Clinical Trials from
Pharma Help line Society, (PDRCT) Jaipur. Attended as
subject evaluator for poster presentations for National
seminar organized by MAM College of Pharmacy, Gun-
tur on 8-9th Nov, 2015. Attended as subject evaluator
for oral presentations in National seminar organized
by ASN College of Pharmacy, Tenali, Guntur on 18th
Jun, 2016.Attended as subject evaluator in Medicinal
chemistry oral presentations for Pharmaphoenix-2K13
organized by Victoria College of Pharmacy, Nallapadu,
Guntur, in association with IPA (Education Division, Mum-
bai) on 2nd April 2013. Attended as subject evaluator in
Pharmaceutical Chemistry for poster presentations for
National seminar organized by Acharya Nagarjuna Uni-
versity, Guntur on 9-10th Nov, 2012.Attended as subject
evaluator for poster presentations for National seminar
organized by MAM College of Pharmacy, Guntur on 8-9th
Nov, 2015.Attended as subject evaluator for oral presen-
tations in National seminar organized by ASN College of
Pharmacy, Tenali, Guntur on 18th Jun, 2016. Attended
as subject evaluator in Medicinal chemistry oral presen-
tations for Pharmaphoenix-2K13 organized by Victoria
College of Pharmacy, Nallapadu, Guntur, in association
with IPA (Education Division, Mumbai) on 2nd April
2013. Attended as subject evaluator in Pharmaceutical
Chemistry for poster presentations for National seminar
organized by Acharya Nagarjuna University, Guntur on
9-10th Nov, 2012. Presented a poster presentation enti-
tled “Synthesis and Characterization of new schiff bases
containing Pyridine moiety and their derivatives as anti-
oxidant agents” in UGC sponsored National seminar on
The promise of Green Chemistry, organized by the Dept.
of Chemistry, Andhra Layola College, Vijayawada on Nov
16-17th 2011 and Secure 1st prize.
drpravi.p@gmail.comBIOGRAPHY