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Pharma Summit 2018 & Gastro Summit 2018 Asian Journal of Biomedical and Pharmaceutical Sciences | ISSN: 2249-622X | Volume 8
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Shivakumar Hugar et al., Asian J Biomed Pharmaceut Sci 2018, Volume 8 | DOI: 10.4066/2249-622X-C5-014
EVALUATION OF ATTENUATING EFFECT OF
COCOS NUCIFERA
ON COLD RESTRAINT
STRESS MODEL IN RATS
Shivakumar Hugar, Virupanagouda P Patil, Nanjappaiah H M
and
Chandrashekhar V M
BLDEA’s SSM College of Pharmacy and Research Centre, India
Objective:
Traditional practitioners commonly use herbal extracts and poly-
herbal formulation for the management of stress. Ranahamsa Rasayanaya,
a polyherbal formulation which contains
Cocos nucifera
is one of the ingre-
dients used in management of stress disorders. Hence, this research was
undertaken to scientifically validate antistress effect of
Cocos nucifera
L. en-
docarp.
Material and Methods:
The test extract was subjected for preliminary phyto-
chemical investigation to detect the presence of type of phytoconstitutents.
The anti-stress effect of graded doses (125, 250 and 500 mg/kg, b/w) of eth-
anolic extract of
Cocos nucifera
endocarp was evaluated using cold restraint
stress model in rats. Its antistress potential was investigated by estimating
various parameters such as hematology, serum biochemical markers such as
glucose, cholesterol, triglycerides and blood urea nitrogen (BUN). The weight
of organs (Liver, spleen and adrenal glands) of experimental animals and nor-
adrenaline and serotonin contents of brain tissue of stressed rats also record-
ed.
Withania somnifera
(100 mg/kg) used as reference standard.
Results and Discussion:
Screening doses of test extract chosen based on the
LD50 cut-off value. The results of preliminary phytochemical investigation of
test extract reveal the presence of flavonoids and tannins. Ethanolic extract
of
Cocos nucifera
endocarp at 250 mg and 500 mg/kg exhibited significant
anti-stress property against cold restraint stress model, which was evident by
significant attenuation of altered haematological, serum biochemical mark-
ers, organs weight and neurotransmitter levels of brain tissue. The result of
125 mg/kg of the test extract was found to statistically not-significant.
With-
ania somnifera
, reference standard drug demonstrated significant anti-stress
effect. The observed significant antistress effect of plant extract could be
attributed to the presence of flavonoids and tannins.
Conclusion:
The findings of the present study justify the incorporation of
Co-
cos nucifera
in Ranahamsa Rasayanaya, a well known classical Sri Lankan
Rasayana drug used in the management of stress disorders.
Shivakumar Hugar working as Professor and Head, Post
Graduate Dept. of Pharmacology, BLDEA’s SSM College
of Pharmacy & Research Centre, Vijayapur – 586103,
Karnataka, India. He obtained PhD degree fromKuvempu
University, Shankaragatta, Shivamogga in the year 2007.
He is having 21 years of experience in teaching and re-
search. He guided 45 M Pharm and 3 PhD students and
01 student of Pharmacology specialization is pursing
PhD under his supervision. Published 56 research papers
in national and international journals. Presented 09 oral
and 55 posters in national and international conferences
and got best research paper award for 3 oral presenta-
tions. Attended International conference organized by
Master Skill University, Malaysia in the year 2011. Shi-
vakumar Hugar was Board of Studies (BOS) member
in Pharmacy (UG) 2009 - 2011, Rajiv Gandhi University
of Health Sciences (RGUHS), Bangalore. Currently he is
member of BOS in Pharmacy (PG) and also faculty mem-
ber of Pharmacy, RGUHS, Bangalore. He has received
research grant from RGUHS, Bangalore. He is also an
editorial board member and reviewer for the few reputed
journals. Shivakumar Hugar has been received Shikshak
Shri Award from chief justice & first vice president of Ne-
pal. This event was organized by Indo-Nepal Antarrashtri-
ya Samrasta Manch on account of teacher’s day on 5th
September at Krishna Menon Bhavan, New Delhi. He was
member of International Organizing Committee of 12th
International congress of Ethnopharmacology, Jadavpur
University, Kolkata, India, held on February 17-19, 2012.
He was the poster evaluator for P4 conference jointly
organized by KLE University & Manipal, held at Belagavi,
Karnataka, India. He is life member of APTI, KSPC, Amer-
ican Botanical Council and ACP. He is an examiner for
various universities and evaluated PhD thesis of different
universities.
shivkumarhugar@yahoo.com.
BIOGRAPHY