pathology-surgical-pathology-2018-posters

9 / 15

Page 35
allied
academies
Journal of Pathology and Disease Biology | Volume 2
September 06-07, 2018 | Edinburgh, Scotland
Pathology and Surgical Pathology
International Conference on
Immunohistochemical expression and microsatellite instability in endometrial carcinoma
Hala Naguib Hosni
Cairo University, Egypt
Background: Endometrial cancer (EC) is the fifth most
common female cancer worldwide constituting 4.8% of
cancer in women. In 2012, around 320,000 new cases of
endometrial cancer were diagnosed worldwide (Ferlay et al.,
2015). EC is a disease of older, postmenopausal women (i.e.,
the sixth and seventh decades of life) and is uncommon in
young women; 2% to14% of endometrial carcinomas occur in
women 40 years of age and younger. Most of these patients
have an identifiable source of excess estrogen, while in a
small subset the pathogenesis is related to mismatch repair
abnormality and Lynch syndrome (Garg and Soslow, 2014).
Mismatch repair (MMR) behave as tumor suppressors and
the most clinically relevant include MLH1, MSH2, MSH6,
and PMS2 (Frolova et al., 2015). MMR results in a strong
mutator phenotype known as microsatellite instability (MSI),
which is a hallmark of Lynch syndrome-associated cancers
(Yamamoto & Imai, 2015).
Aim of the work: To detect the expression of MMR
proteins in endometrial carcinoma cases using the
immunohistochemical (IHC) technique (MLH1, MSH2, MSH6
and PMS2) with correlation to different clinicopathologic
parameters.
Material and methods: In this study, the pathology files
at the Pathology Department, Kasr Al Ainy Hospitals and
Ahmed Maher Teaching Hospital were reviewed to randomly
60 endometrial carcinoma cases. Five-micron thick sections
stained with hematoxylin and eosin (H&E) and MLH-1, MSH-
2, MSH-6 and PMS-2 immunostains. Loss of MLH1 and PMS2
was interpreted as a likely abnormality in MLH1, whether by
germline defect or epigenetic mechanism whereas isolated
loss of PMS2 was considered likely due to a germline PMS2
mutation. Similarly, concurrent loss of MSH2 and MSH6
suggested an MSH2 germline defect, whereas isolated loss
of MSH6 was suggestive of mutations in MSH6 alone.
Results: A statistically significant relationship exists between
MMR IHC proteins and tumor grade. However, a statistically
insignificant correlation was found between MMR IHC
proteins and the age of patients; tumor histopathological
types and FIGO stage.
e: