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Journal of Pathology and Disease Biology | Volume 2

September 06-07, 2018 | Edinburgh, Scotland

Pathology and Surgical Pathology

International Conference on

Study of megakaryocytic morphology by digital morphometry in bone marrow biopsy specimens in

hematological diseases

Rajat Bajaj, Raman DK, Venkatesan S, Sharma S

and

Bharadwaj R

Armed Forces Medical College, India

Introduction & Background:

Megakaryocytes are unique

and dynamic cells which produce platelets by cytoplasmic

fragmentation. They are affected in a variety of hematological

conditions. A defect in any stage of megakaryocytopoiesis

can lead to dyspoietic megakaryocytes or thrombocytopenia.

This mandates the need to assess them qualitatively and

quantitatively. Digital morphometric analysis can be used

to precisely quantify the megakaryocytic morphology

with respect to area, nuclear size, nuclear cytoplasmic

ratio, nuclear roundness factor, nuclear contour ratio.

Material and Methods:

Hematoxylin & Eosin (H&E),

Immunohistochemistry (IHC) stained sections of bone marrow

biopsies were evaluated for megakaryocyte morphology

and computer assisted digital morphometry. High resolution

photomicrographswere taken for all cases and aminimumof 10

megakaryocytes were evaluated for each case. The cytoplasmic

and nuclear delineation was done manually and precise

measurements of cell area, perimeter, nuclear size, shape,

nucleustocytoplasmratioandimportantindiceswereevaluated

by computer assisted digital morphometry and correlated.

Results:

170 Bone marrow biopsies were studied which

included myeloproliferative neoplasms (MPN) namely chronic

myeloid leukemia, Polycythemia Vera, Essential thrombocytosis

and Myelofibrosis; Idiopathic thrombocytopenic purpura,

Myelodysplastic syndrome, megaloblastic anemia, plasma

cell neoplasms and remission marrows post chemotherapy.

Statistically significant morphological differences were

seen in various hematological groups with regards to cell

count, morphology, N:C ratio, nuclear and cytoplasmic

perimeter, nuclear and cytoplasmic roundness. IHC (Anti

CD 61) was useful in highlighting the megakaryocytes

which were missed on H&E especially in MPN’s.

Conclusion:

Megakaryocytes show significant quantitative

and qualitative variations in various haematological disorders,

especially myeloproliferative neoplasms. Objective evaluation

and classification of megakaryocytes in these disorders may be

useful in arriving at an early and a more accurate diagnosis. The

morphometric parameters need to be reinforced and validated

by a larger study to objectively classify the megakaryocytes.

drrajatrbajaj@gmail.com

Notes: