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J Med Oncl Ther 2017 Volume 2 | Issue 3

International Conference on

Oncology and Cancer Therapeutics

October 30- November 01, 2017 | Chicago, USA

Double rolling circle replication (DRCR): A mode of amplification of oncogene as well as drug-resistant

genes and replication of HSV and chloroplast DNA

Takashi Horiuchi

Tokai University School of Medicine, Japan

t is well established that eukaryote nuclear chromosomes

are duplicated from multiple origins of replication. It

remains a mystery, however, how genomes of some viruses,

such as HSV (Herpes simplex virus) and Baculovirus, or

chloroplasts, are replicated. We have found recently that

(i) double rolling circle replication (DRCR), originally found

responsible for replication of yeast 2µ plasmid DNA, can lead

toamplificationof oncogenes aswell asdrug resistancegenes,

and (ii) that DRCR is highly recombinogenic. In addition, we

will present our model, based on these findings, that DRCR

is involved in DNA replication of HSV-1, chloroplasts and

some mitochondria. The model could explain how DRCR

contributes to replication-recombination coupling of HSV,

and also how it promotes amplicon shortening during gene

amplification.

Speaker Biography

Takashi Horiuchi has received his BS degree in 1969 from Department of Agriculture,

Kyoto University, and Kyoto, Japan. He did his MS degree in 1971 from Department

of Agriculture, Kyoto University and another MS degree in 1973 from Department of

Science, Kyoto University. He completed his PhD degree in 1980 from Department of

Science (Institute for Virus Research), Kyoto University. He received Kihara Prize for

“Identification and characterization of DNA replication fork blocking event”.