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J Med Oncl Ther 2017 Volume 2 | Issue 3
International Conference on
Oncology and Cancer Therapeutics
October 30- November 01, 2017 | Chicago, USA
Identification of canine papillomavirus in the transmissible venereal tumor using the polymerase
chain reaction technique in canines (Canis lupus familiaris)
Sergio Ayala-Díaz
1
, Joaquín Manzo-Merino
2
, Marcela Lizano
2
and
Jaime Arroyo Ledezma
1
1
Universidad del Mar, México
2
Instituto Nacional de Cancerología, México
T
he Venereal transmissible tumor (TVT), also known as
infectious sarcoma, venereal granuloma, transmissible
lymphosarcoma or Sticker´s sarcoma, is a neoplastic
disease affecting dogs and its propagated mainly during the
intercourse. The TVT is located mainly in the genital area
with a lower frequency at the oral cavity, nasal cavity, eyes
and skin. The disease is presented as a tumoral mass at the
glans bulb in males, and in the vaginal vestibule. Up to date,
there is no evidence for a viral agent as the causative agent
for TVT development. The present work was aimed to analyze
21 samples from canines with TVT for clinical, cytological
and histopathological evaluation, as well as for blood count,
clinical chemistry, bacterial culture and molecular analysis to
identify papilloma virus DNA sequences. Clinical diagnostic
confirmed the clinical and biochemical features for TVT and
molecular analysis demonstrated the viral DNA presence in
the samples through the amplification of the viral sequence L1
(major capsid coding gene of papilloma virus) using different
primer sets, the MY primers amplified a 450 bp band in seven
out of 23 samples (33%). L1 positive samples were sequenced
to analyze the identity of the PCR product. The PVF and Fap-
64 primer set, targeting the L1 sequence of Canine Papilloma
Virus (CPV), showed positivity in 16 out of 21 samples
(76%). On the other hand, the amplification using the CP4/5
primer set, aimed to amplify the E1 region of CPV, showed
no amplification at all. These results support the possible
causative association between CPV and TVT; nevertheless,
confirmatory studies are required to confirm such as
statement. This work represents the first evidence indicating
that a viral agent might be involved in the pathogenesis of TVT
with high impact in the understanding of TVT pathogenesis.
Speaker Biography
Sergio Ayala-Díaz is graduated in Zootechny from Universidad del Mar, holds a degree
in Hematology from the Universidad Nacional Autónoma de México and a Master
of Science from Universidad del Mar in collaboration with the Instituto Nacional de
Cancerología, México. He has worked in collaboration with other researchers in the
area of Epidemiology and Molecular Biology of oncogenic viruses and transmissible
tumors such as canine transmissible venereal tumor to generate timely diagnostic
tools.
e:
blo_gun@hotmail.com