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J Med Oncl Ther 2017 | Volume 2 Issue 4
Oncology and Biomarkers Summit
November 27-28, 2017 | Atlanta, USA
Annual Congress on
T
he effects of aqueous stem extract of
Adenia
cissampeloides
on selected liver function biomarkers of
fish (
Clarias batrachus
) were investigated. The aims were to
determine the lethal concentration (LC50) of the extract to
the fish and the effects on Aspartate aminotransferase (AST),
Alanine aminotransferase (ALT), Alkaline phosphatase (ALP)
and Unconjugated Bilirubin (UB). A total of one hundred and
sixty (160) fish of average weight of 122 g were used in the
study and grouped into five (A, B, C, D and E). The 24 hr, 48
hr and 72 hr lethal concentration (LC50) of the stem extract
were determined. Those for the assay were exposed to 00
g/l, 0.6250 g/l, 1.250 g/l, 2.50 g/l and 5.0 g/l concentrations
respectively, in triplicate for a total of eight hours. Blood
sample was collected from one fish picked from each group
at one-hour interval and assayed. One factor completely
randomized ANOVA design was adopted in the analysis.
The 24 hr, 48 hr and 72 hr, LC50 were 5.00 g/l, 2.50 g/l and
2.50 g/l respectively. There were increases in the activities
of all the parameters assayed for. The results of analysis
showed significant (p<0.05) increases in AST activities and
concentrations of UB. However, increases in the activities
of ALT and ALP were not significant (p>0.05). Large effect
size (ω2) of 0.42 and 0.52 for UB and AST, respectively,
were obtained. AST/ALT ratio of 1:5 indicated damages to
liver cells and disruption of vital processes that might have
elicited unfavorable cytotoxic reactions in the fish. It is
possible that the same effects may occur in man; therefore,
it was recommended that fish killed with this plant should be
avoided if not properly heat treated.
e:
emejiemmanuelo@yahoo.comToxicological studies of aqueous extract of
Adenia cissampeloides
in
Clarias batrachus
fish
Emmanuel Emeji
Ebonyi State College of Education, Nigeria