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Microbiology: Current Research 2017
Volume 1 Issue 2 Microbes Infection 2017
Notes:
Page 22
September 28-29, 2017 | London, UK
Microbes Infection
38th Annual congress on
Antiviral activity and possible mechanisms of action
of Aristolochia bracteolate against influenza A virus
Mona Timan Idriss1, Malik SulimanMohamed 2, Sarawut Khongwichit 3,
Natthida Tongluan3, Duncan R Smith3, N H Abdurahman4 and Alamin Ibrahim
Elnima2
1Sudan International University, Sudan
2 Khartoum university, Sudan
3Mahidol University, Bangkok, Thailand
4Universiti Malaysia Pahang, Malaysia
We investigated the anti-influenza virus activity of
Aristolochia bracteolate and possible mechanism(s)
of action in vitro. We found that Aristolochia bracteolate has
anti-influenza-virus activity, and both pre-incubation of virus
prior to infection and post-exposure of infected cells with
Aristolochia bracteolate extract significantly inhibited virus
yields. Influenza-virus-induced hemagglutination of chicken
red blood cells was inhibited by Aristolochia bracteolate
extract treatment, suggesting that Aristolochia bracteolate can
inhibit influenza A virus infection by interacting with the viral
hemagglutinin. Furthermore, Aristolochia bracteolate extract
significantly affect nuclear transport of viral nucleoprotein
(NP). To best of our knowledge, this study revealed for the
first time that Aristolochia bracteolate extract can inhibit both
viral attachment and replication and offers new insights into
its underlyingmechanisms of antiviral action.The whole plant
of Aristolochia bracteolate collected from Sudan and Extracted
with 70% methanol. The crude extract was screened for its
cytotoxicity against MDCK cell line by Presto- Blue assay and
WST-1 assay. Antiviral properties of the plant extract were
determined by cytopathic effect inhibition assay and virus
yield reduction assay (plaque assay). Time of addition assay,
and nuclear export mechanism were also performed.
Biography
Mona Timan Idriss is a Lecturer of Microbiology in the Department of
Pharmaceutical Microbiology, Faculty of Pharmacy, Sudan International
University, in Sudan. She is currently pursuing PhD studies in Molecular Virology
(development of novel antiviral drugs from Sudanese plants and possible
mechanism of action). She also worked as a Visiting Scientist in the department
of Bimolecular Sciences, University of Mahidol, Thailand. She participated in
many projects with members of the Molecular virology laboratory in University
of Nagasaki, Japan using molecular biology techniques. Most recently, she has
written two papers in virology research. She is selected as an Editorial Board
Member for Immunotherapy Research Journal and SciFed Journal of Mycology.
Mona Timan Idriss et al., Microbiology: Current Research 2017