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Microbiology: Current Research 2017
Volume 1 Issue 2
Microbes Infection 2017
Notes:
Page 18
September 28-29, 2017 | London, UK
Microbes Infection
38
th
Annual congress on
Molecular characterization of methicillin resistance
gene among Staphylococcus aureus isolated from
poultry farms in Kaduna, Nigeria
Josiah Ademola Onaolapo
1
, S K Parom
3
, J C Igwe
2
, R O Bolaji
1
and
G O
Adeshina
1
1
Ahmadu Bello University, Nigeria
2
Kaduna State University, Nigeria
3
Gombe State University, Nigeria
Statement of Problem
: Zoonotic transfer of resistance genes
has been reported as one of the major causes of increased
community associated methicillin resistance S.
aureus
(CAMRSA), which has contributed to high hospital visit,
mortality and morbidity in clinic.
Aim
:This study evaluates the occurrence of S.
aureus
encoding
MecA gene in poultry birds from Kaduna metropolis.
Methodology
: sample collection was carried out using
standard epidemiological procedure, S. aureus isolation,
identification and biochemical test were carried out using
standard microbiological methods, antibiotic susceptibility
testing was carried out using disc diffusion agar while
molecular analysis was carried out using PCR techniques.
Results
: A total of 600 poultry samples from 300 layers and
300 broilers were randomly collected from 4 poultry farms
for evaluation. Using Microgen biochemical kit 27.3% of
the samples collected yielded S. aureus. Using disc diffusion
method 37.2 % (61) of the S.
aureus
were resistant to oxacillin.
The resistance profile of the oxacillin resistant isolates showed
that the isolates were highly resistant to tetracycline (88.5%),
ciprofloxacin (80.3%), mildly resistant to cotrimoxazole
(32.8%), vancomycin (31.1%) and susceptible to amoxiclav
(93.4%), cefoxitin and gentamicin (97.7% respectively). High
percentage of the isolates 34.4% (21) harbored the
MecA
gene that amplified at 162 base pair while none of the isolates
harbor MecA gene with 500bp.
Conclusion
: This study reports the presence of MDR
S.
aureus
encoding
MecA
gene among S.
aureus
isolates
evaluated from poultry farm in Kaduna metropolis, hence
this calls for concern as poultry products serves as means
to fast dissemination of livestock and community associated
methicillin resistant S.
aureus
as high percentage of poultry
farmers, abattoirs and meat vendors carries out their activity
without veterinary nor government control.
Biography
J A Onaolapo is a Professor of Pharmaceutical Microbiology at the Department
of Pharmaceutics and Pharmaceutical Microbiology, Faculty of Pharmaceutical
Sciences, Ahmadu Bello University, Zaria Nigeria. He rose from the post of an
Assistant Lecturer in 1979 to the rank of a Professor in 1996. He had BSc in
Pharmacy with Hon. Second Class Upper division, ABU, Zaria in the year 1977;
MSc in Pharmaceutics, ABU, Zaria, 1982 and a PhD in Pharm. Microbiology,
Aston University, Birmingham, UK in 1986. His area of focus has been bacterial
drug resistance. He has published over 200 scientific articles both in local
and international journals. He has supervised over 30 PhD students in the
field of Pharmaceutics and Pharmaceutical Microbiology, over 50 MSc and
100 undergraduate students. He has also carried out researches on finding
antibacterial drugs from natural products.
jaonaolapo@gamail.comJosiah Ademola Onaolapo et al., Microbiology: Current Research 2017