microbes-infection-2017-scientifictracks-abstracts

7 / 16

alliedacademies
Microbiology: Current Research 2017
Volume 1 Issue 2 Microbes Infection 2017
Notes:
Page 19
September 28-29, 2017 | London, UK
Microbes Infection
38th Annual congress on
Pseudomonas exoenzyme y-mediated evasion of host
innate immune responses
Un-Hwan Ha
Korea University, Republic of Korea
Pseudomonas aeruginosa is recognized as a leading
cause of respiratory infections in cystic fibrosis or in
immunocompromised patients. P. aeruginosa possesses
a number of virulence factors released through diverse
secretion systems, and type III secreted effectors have
obtained much attention for their ability to manipulate host
cell function and viability during infections. However, little
is known about the impact of exoenzyme Y (ExoY), which
is directly translocated into the cytoplasm of infected host
cells, on the modulation of host innate immune responses.
In this study, we analyzed effects of ExoY in the activation
of inflammasome, which results in IL-1 production and
pyroptotic cell death. Inflammasome-mediated production
of IL-1 and formation of pyroptotic cell death were clearly
reduced in response to ExoY. These suppressive effects were
mediated by the adenylate cyclase activity of ExoY, which
plays a role in delaying the activation of NF-B and caspase-1,
a key component of inflammasome-mediated responses.
Moreover, the reduction in cytotoxicity was in part associated
with ExoY-involved suppression of bacterial motility, which
probably causes the reduction of bacterial contact with cells.
Together, these results demonstrate that ExoY can influence
both host and bacterium itself to reduce inflammasome-
related responses by delaying the activation of inflammatory
pathways and suppressing bacterial motility.
Biography
Un-Hwan Ha has completed his PhD in the field of microbiology and Immunology
from the University of Florida in 2002 and has continued postdoctoral studies in
the field of innate immunity and cellular microbiology from House Ear Institute
and University of Rochester Medical Center. In 2008, he got an Assistant
Professor position at the Department of Biotechnology and Bioinformatics,
Korea University and has served as Professor since 2015. He has published
about 20 research articles contributed as a corresponding author in reputed
journals since 2008. His main research area is host-microbe interactions by
aiming to understand both bacterial pathogenesis and host innate immune
responses.
Un-Hwan Ha, Microbiology: Current Research 2017