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Microbiology: Current Research 2017
Volume 1 Issue 2 Microbes Infection 2017
Notes:
Page 33
September 28-29, 2017 | London, UK
Microbes Infection
38th Annual congress on
Probiotics for the use in the development of cattle
production in Kuwait
Tahani Al-Surrayai
Kuwait Institute for Scientific Research, Kuwait
Dairy farms in Kuwait are facing extremely arid conditions
that induce animal stress and tend to reduce energy
reserves that weaken the immune system making the animal
more susceptible to disease and increase animal mortality. To
combat these diseases, farmers administer antibiotics to the
cattle which are also used by humans. Overuse can lead to the
bacteria developing resistance to these antibiotics. In addition,
when dairy products from these antibiotic-ingested cattle
are consumed by humans, the antibiotic residue enters their
system causing them to develop resistance to those antibiotics.
The main objectives of this research project were to isolate
and evaluate probiotic lactic acid bacteria (LAB) during four
seasons for utilization as an alternative to antibiotics in cattle
production to control enteric pathogens, enhance productivity
and improve food safety. The evaluation was covered by
determination of antagonistic activity of LAB using in vitro
tests and determining their tolerance to acidic pH, resistance
to bile salts, resistance to antibiotics bacteriocin production,
and aggregation and co-aggregations. The preliminary
isolation process resulted in the isolation of 263 presumptive
Lactobacilli, and among them, 80 were confirmed to belong
to the lactic acid bacteria group by means of molecular tools
(16S rRNA-Polymerase Chain Reactions (PCR)-sequencing).
Ten representative strains were chosen and screened for their
probiotic potential. During this study, active LAB strains were
isolated such as L. fermentum, L. rhamnousus and L. reuteri.
These strains can potentially inhibit the growth of some
common pathogen (S. enterica and E. coil ) and tolerate the
acidic condition in the ruminant’s digestive system, tolerate
bile salt and have the ability to adhere to hydrocarbons. As
a final result, a pool of 4 strains seemed to have the relevant
probiotic potential to be further tested as agents able to reduce
bacterial infections.
Biography
joined the Biotechnology Dept. at KISR in 1995 as a Research Assistant. During
the past 22 years, she developed excellent technical skills and has actively
participated in several important research projects in the area of Environmental
Biotechnology. Her role in the many projects resulted successfully in the
isolation, characterization and optimization of a large number of new microbial
strains from the local environment. These strains have been used as an integral
part of the developed sulfur amendment for enhancing soil fertility. Additionally,
she leads a task in a study that focused on the screening and evaluation of
PAHs degrading microorganisms for the local environment. Besides her depth
of knowledge and skills in biochemistry and microbiology, she is involved in the
development of probiotic bacteria for use in livestock and poultry. Accordingly,
she has been leading two successful projects in probiotics fields for livestock.
Tahani Al-Surrayai, Microbiology: Current Research 2017