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Microbiology: Current Research 2017
Volume 1 Issue 2 Microbes Infection 2017
Notes:
Page 16
September 28-29, 2017 | London, UK
Microbes Infection
38th Annual congress on
Investigating the prevalence of rotavirus and its
early detection in calves of Kuwait dairy farms using
molecular techniques
Mohammad Alotaibi and Sami Al Amad
Kuwait Institute for Scientific Research, Kuwait
Statement of the Problem: Rotavirus is one of the main
pathogens causing morbidity and mortality in neonatal dairy
calves worldwide, and is responsible for 30-44% loss in cattle.
It is the most common etiologic agent of diarrhoea in neonatal
dairy calves and children, the dominant type being group A.
Another impact of the disease is the massive financial loss
in the livestock industry. In Kuwait, viral diseases are the
major cause of high mortality and morbidity rates in young
dairy calves. The proposed study is intended to assess the
prevalence of rotavirus in dairy calves by applying molecular
diagnostic techniques.
Methodology: This project is designed to compare molecular
with immunological diagnostic methods for the early
detection of rotavirus in calves in Kuwait. Evaluation of
detection methods for viral particles and ribonucleic acid
(RNA) was performed using latex agglutination (LTA) and
reverse transcriptase-polymerase chain reaction (RT-PCR).
Findings: A total number of 270 fecal and 10 water samples
were collected from farms Sulaibiah and Kabd. The fecal
samples in triplicate from calves under one year of age based
on age and gender. The foecal samples were suspended
in phosphate buffer saline (PBS) and tested with Latex
Agglutination and 14% of samples showed presence of
rotavirus, while RT-PCR showed 30% of rotavirus. These
findings indicate that the RT-PCR assay is more specific and
sensitive and can be effectively used for the early detection of
rotavirus in foecal calf samples.
Recommendations: The following are the recommendations
for early detection of rotavirus in calves: 1. isolation of
infected calves to prevent spread of rotavirus between herds.
2. application of rotavirus vaccine to minimize the chances
of claves for infection, and 3. application of appropriate
diagnostic method by farmers and livestock companies to
investigate the presence of rotavirus instantly and take the
required actions immediately.
Biography
Dr. Mohammad Alotaibi, graduated from University of Leicester in the UK,
is an expert in pathogenic food-borne viruses who is a researcher in Kuwait
Institute for Scientific Research . He is also an expert
in inactivation of pathogenic microbes including viruses and bacteria using
solar irradiation of water especially in places under natural crises where water
sanitation infrastructure is destroyed. He has published many original scientific
papers in international journals. Dr. Alotaibi has ongoing research projects
including the research that will be presented in the conference for the early
detection of rotavirus in calves in Kuwait and its impact for the morbidity and
mortality of this virus.
Mohammad Alotaibi et al., Microbiology: Current Research 2017