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Journal of Systems Biology & Proteome Research | Volume 2
J u n e 2 5 - 2 7 , 2 0 1 8 | D u b l i n , I r e l a n d
MASS SPECTROMETRY
AND PROTEOMICS
International Conference on
Tarfa M Peter et al., J Syst Biol Proteome Res 2018, Volume 2
THE ROLE OF PROGRAMMED
CELL DEATH ‘APOPTOSIS’ IN THE
DEVELOPMENT OF INNER SULCUS IN
THE COCHLEA
Tarfa M Peter1 and Paul M Leonard2
Gombe State University, Nigeria
Hearing loss is one of the most common chronic diseases that affect both
young and old but it is most prevalent in old people. This condition is gen-
erally irreversible in humans and can be due to the loss of hair cells, which are
unable to regenerate. However, recent evidence of some regenerative ability
reported in a number of non-mammal vertebrates have given us hope that,
in the future a solution may be discovered. Although several advances have
been recorded in this field in recent times and ere are still challenges ahead.
This study tried to investigate he formation of the inner sulcus located in the
cochlea, as it is thought that, the processes involved during the development
of this important region are most likely due to apoptosis or another type of
programmed cell death, although this has not yet been confirmed. Mouse
expressing an EGFP (green fluorescent protein) reporter at the Tecta locus
was used. Specimens were stained with phalloidin as a general cell stain of
f-actin and this was combined with (Terminal deoxynucleotidyl transferase
dUTP Nick End) TUNEL staining in order to observe whether dying cells are
the result of programmed cell death. Very little TUNEL staining was observed
in the developing sulcal region, although some were seen in the associated
mesenchymal cells in the cochlea. In some of the sections, Blebbing as well
as extrusion of some cells that are thought to be undergoing programmed cell
death were evident during the formation of the sulcus. The formation of the
sulcus occurs earlier in the basal region of the cochlea than in the apical part
following the regression of the greater epithelial ridge (GER) cells. Counting
of nuclei in the sulcal region during the formation suggest that cells are being
lost. It is not easy to establish whether these cells that are being removed
could be due to apoptosis or another type of programmed cell death.
Tarfa M Peter has completed his MSc. from
University of Maiduguri, Borno State, Nigeria.
He is the Histology lecturer, in the Department
Human Anatomy, Gombe State University. He
has published more than 10 papers in reputed
journals and is presently undergoing his Ph. D in
Ahmadu Bello University Zaria.
BIOGRAPHY