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allied
academies
August 16-17, 2018 | Copenhagen, Denmark
Industrial Biotechnology and Bioprocessing
International Conference on
Journal of Archives of Industrial Biotechnology | Volume 2
Biodegradability of microbial synthesized Poly-β-Hydroxy-butyrate produced from
Pseudomonas
aeruginosa
Dw7 local isolate
Nibras M Al-hasan
1
, Iman H Gatea
1
, Saad H Khudei
1
, Nadhem H Hayder
2
and
Marwa Sh Mahmood
2
1
Ministry of Science and Technology, Iraq
2
Baghdad University, Iraq
T
he environmental pollution by petro based plastics a
cause of concern, which are non-biodegradable. Hence
biodegradable and biologically synthesized polymers
with similar properties of conventional plastic are sought.
Poly-β-hydroxy-butyrate (PHB) is a member of a family of
polyhydroxyalkonates synthesized by numerous bacteria as
an intracellular carbon and energy storage compound under
nutrient-limiting conditions with excess carbon.The use of
biodegradable polymers has been increasing in recent years,
specifically toward various biomedical applications as these
materials not only serve the desired purpose but also get
eliminated from the body due to their biodegradable nature.
Pseudomonas aeruginosa Dw7 local isolate was identified as
PHB producing isolate in a previous study. Important properties
of the product were studied.
The biodegradability studies of PHB were concluded in solid,
liquid and in soil environment using the fungus Aspergillus
niger which was tested for the degradation of PHB in assay
agar medium as well as in liquid cultures was concluded using
Aspergillus niger isolate. The contribution of A niger to the
microbial degradation of PHB film in soil was studied. The clear
zone around the colonies was measured to evaluate the activity
of this isolate; it was determined by recording 7 mm of clear
zone around the fungal colonies in 7 days of incubation at 30°C.
Relatively after 12 days, PHB showed a high ability to degrade,
since A niger produces the highest biomass 2.03 g/l leaving less
residual PHB in the medium which recorded 16%. The results
showed that 100% of degradation after 40 days indicating the
high loss in PHB content in the soil as an open system. One of
the promising and recently applications of PHB are antimicrobial
agent. Antibacterial activity test was carried out using disk
diffusion method against gram negative and positive bacteria.
The results showed that antibacterial compounds of PHB which
was extracted by hypochlorite solution and chloroform are
active against selected bacterial isolates showed inhibition zone
ranging between (8-40mm) as indicator of antibacterial activity.
e:
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