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Page 26

allied

academies

August 16-17, 2018 | Copenhagen, Denmark

Industrial Biotechnology and Bioprocessing

International Conference on

Journal of Archives of Industrial Biotechnology | Volume 2

Studies on fermentative production of Beta-carotene from

Phaffia rhodozyma

Bibhu Prasad Panda, Shweta Nagal

and

Afreen Raja

Jamia Hamdard, India

P

lating of the astaxanthin-producing yeast Phaffia rhodozyma

on yeast-malt agarmedia containing different concentration

of Beta-Ionone gave colonieswith yelloworangepigment after 6

to 8 days of fermentation. Isolation of Beta-Carotene producing

colonies were done, followed by testing for pigment production

in shake flasks, demonstrated that pigment concentration were

increased two-to five fold for Beta-Carotene content compared

with the parental one. It was found from our study that Beta-

Carotene production was improved by addition of Beta-Ionone

at concentration of about 10

-4

and 2×10

-4

after sixth day of

fermentation. By using different carbon and magnesium source

yield of Beta-Carotene content could be increased to higher

level with sucrose 283.01 µg/g, ammonium sulphate (234.80

µg/g) and magnesium sulphate (218.65 µg/g)

e

:

bibhu_panda31@rediffmail.com