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Allied J Med Res 2017
Volume 1 Issue 2
Herbal Medicine 2017
September 01-02, 2017 London, UK
3
rd
International Conference and Expo on
Herbal & Alternative Medicine
Page 60
Fertility regulating effects of Withaferin-A and
Withanolide-A: a treatment in male albino rats
Pratap Chand Mali
University of Rajasthan, India
Background:
Population explosion is creating immense
pressure on natural non-renewable resources leading to
economic imbalances, environment pollution and health
hazards thus human fertility regulation becomes a global
concern. However, a wide variety of contraceptives are
available for fertility control to both men and women,
although, caused some side effects in users. In recent
years, use of traditional and alternative medicine has
increased worldwide; number of modern drugs has been
developed from natural sources, particularly plants, based
on their use in traditional medicine.
Objective:
Two Withanolide i.e. Withanolide-A and
Withaferin-Awere administered orally at the three different
dose levels for 60 days to in adult male Wistar rats to
evaluate effects on reproduction and fertility to develop
a safe, orally effective and reversible male contraceptive
agent.
Materials & Methods:
Withaferin-A and Withanolide-A
were separated from Withania somnifera dissolved in
DMSO to administered orally. The initial and final body
weights of the animals were recorded. Testes, epididymis,
seminal vesicles and ventral prostrate were dissected out,
freed from adherent tissues and weighed to the nearest
milligram. Sperm motility and density were done by routine
procedure. To check fertility of animal mating was carried
out with all the animals 5 days prior to sacrifice. The
mated females could complete the gestation. The number
of pups was recorded and litter size and percent fertility
was calculated. Serum was separated and stored at 20°C
for protein, cholesterol and triglycerides. Serum was
analyses for the estimation of testosterone, FSH and LH
levels by ELISA Kits. Protein, glycogen, cholesterol, sialic
acid ascorbic acid and fructose were estimated in right
side of testis and other accessory reproductive organs.
Sections of testes were stained with Harris’s hematoxylin
and eosin to observe under light microscope. Data are
expressed as mean ± SE and analyse for statistical
significance by using student’s t-test. The study was carry
out under the supervision of the ethical committee of the
Department of Zoology, University of Rajasthan, Jaipur,
India and CPCSEA guidelines followed for maintenance
and use of the experimental animals.
Results:
The weight of testes, epididymites and sex
accessory reproductive organs were significantly
decreased. Sperm density and motility and fertility
of treated male rats reduce significantly. The level of
fructose, protein, ascorbic acid and sialic acid contents
of reproductive tract were significantly declined after the
treatment of both Withanolide. Level of LH, FSH and
testosterone were significantly decreased in Withaferin-A
and Withanolide-A treated rats. Histological observations
showed degenerative changes in germinal epithelium of
testes as compared to control rats.
Conclusions:
The decreased number of spermatocytes,
spermatids and spermatozoa in lumen of seminiferous
tubules after the treatment of Withaferin-A and
Withanolide-A in rats might be due to suppression of
androgen, resulted reduction of fertility potential of
rats. The possible mechanism of antispermatogenic
and antiandrogenic effects of the Withaferin-A and
Withanolide-A treatment are discussed.
malipc_zool@yahoo.co.inAllied J Med Res 2017