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Allied J Med Res 2017
Volume 1 Issue 2
Herbal Medicine 2017
September 01-02, 2017 London, UK
3
rd
International Conference and Expo on
Herbal & Alternative Medicine
Page 57
Antiplasmodial activity of methanolic
leaf extract of mangrove plants against
Plasmodium berghei
Muhaimin
1
, Riski Dwimalida Putri
1
, Andita Utami
1
and Josephine
Elizabeth Siregar
2
1
University of Jambi, Indonesia
2
Eijkman Institute for Molecular Biology, Indonesia
Objective:
Malaria is a mosquito-borne disease caused
by parasitic protozoa from the genus of Plasmodium. The
protozoans have developed resistance against many of
current drugs. It is urgent to find an alternative source
of new antimalarial agent. In the effort to discover new
antimalarial agents, this research has been conducted
on Jambi mangrove plants. This study was conducted to
evaluate the antiplasmodial properties of Jambi mangrove
plants.
Material & Methods:
This study has been conducted
by screening antiplasmodial bioactivity of methanol leaf
extract of mangrove plants against Plasmodium berghei.
Antiplasmodial activity from methanol leaf extract of
mangrove plants, namely
Sonneratia alba
,
Acanthus
ilcifolius
, and
Sonneratia caseolaris
were tested ex vivo
against Plasmodium berghei strain ANKA infected into
Balb-C mice. Antiplasmodial activity were observed by
calculating the parasitaemia percentage of Plasmodium
berghei in mice red blood cells at 0 and 24 hours and
expressed as decreased levels of parasitaemia and
percent of inhibition.
Results:
Based on the decrease of parasitaemia level
and the percent of inhibition value, the three methanol
leaf extracts of mangrove plants (
Sonneratia alba
,
Acanthus ilcifolius
, and
Sonneratia caseolaris
) concluded
to have potential antimalarial activity. The highest activity
showed by the methanol leaf extract of
Sonneratia alba
with percent inhibition as much as 48.5; 44.9; 42.7
and 18.8% at concentrations of 300, 100, 10 and 3 μg/
ml. Antiplasmodial activity can also be studied from the
inhibition of the development life cycle of plasmodium.
Methanol leaf extract of
Sonneratia alba
have activity in
inhibiting the development ring stage to which schizonts
were not found at the extract concentration of 300 μg/
mL. At lower concentration, trophozoite and schizonts still
survived with defect morphological conditions.
Conclusion:
The results of this study indicated that
mangrove plant
Sonneratia alba
was a potent source of
natural antimalarial drugs.
muhaimin_73@yahoo.deAllied J Med Res 2017