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Journal of Industrial and Environmental Chemistry

|

Volume 2

GREEN CHEMISTRY &

TECHNOLOGY

7

th

International Conference on

J u n e 1 8 - 2 0 , 2 0 1 8 | D u b l i n , I r e l a n d

Hao Fang, J Ind Environ Chem 2018, Volume 2 | DOI: 10.4066/2591-7331-C1-002

SINGLE CELL OIL PRODUCTION FROM

AGRICULTURAL WASTES AND ITS

CHEMICAL TRANSFORMATION FOR

PRODUCTION OF LONG CHAIN Α,Ω-

DICARBOXYLIC ACIDS

Hao Fang

Northwest A&F University, China

S

ingle cell oil (SCO) production from lignocelluloses by oleaginous

microorganisms is still high in production cost, making the subsequent

production of biofuels inviable economically in such an era of low oil

prices. Therefore, how to upgrade the final products of lignocellulose-

based bioprocess to more valuable ones is becoming a more and more

important issue. Differently sourced cellulases were compared in the

enzymatic hydrolysis of the steam-exploded corn stover (SECS) and the

cellulasefromthemixedcultureof

Trichodermareesei

and

Aspergillusniger

was found to have the highest enzymatic hydrolysis yield 86.67±4.06%.

Three-stages enzymatic hydrolysis could greatly improve the efficiency

of the enzymatic hydrolysis of SECS, achieving a yield of 74.24±2.69%

within 30 h. Different bioprocesses from SECS to SCO were compared

and the bioprocess C with the three-stages enzymatic hydrolysis was the

most efficient, producing 57.15 g dry cell biomass containing 31.80 g

SCO from 327.63 g SECS. An efficient and comprehensive process from

corn stover to long chain α,ω-dicarboxylic acids (DCAs) was established

by employing self-metathesis, capable of producing 6.02 g long chain

DCAs from 409.54 g corn stover and 6.02 g alkenes as by products. On-

site cellulase production by the mixed culture of

T. reesei

and

A. niger

is proven the most efficient in providing cellulase to the lignocellulose-

based bioprocess. Three-stages enzymatic hydrolysis was found to have

very good application value in SCO production by

Trichosporon cutaneum

from SECS. A whole process from corn stover to long chain DCAs via a

combination of biological and chemical approaches was successfully

established and it is an enlightening example of the comprehensive

utilization of agricultural wastes.

Hao Fang obtained his PhD degree in 2014 at

the Department of Biochemical Engineering,

Zhejiang University, China. Then, he joined Na-

tional Engineering Laboratory of Cereal Fermen-

tation Technology, Jiangnan University, China as

Senior Lecturer. In 2016, he moved to College of

Life Sciences, Northwest A&F University, China

as Associate Professor. His research interests

include Biochemical Engineering, Industrial

Microbiology and Biotechnology and Green

Bio-manufacturing. He has published more than

20 journal papers.

fanghao@nwafu.edu.cn

BIOGRAPHY