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J Pharmacol Ther Res 2017 Volume 1 Issue 2
November 02-03, 2017 Chicago, USA
4
th
International Congress on
International Conference and Exhibition on
Drug Discovery, Designing and Development
Biochemistry, Molecular Biology: R&D
&
Regulation of the activity of the promoter of RNA-induced Silencing, C3PO
Suzanne Scarlata
Worcester Polytechnic Institute, USA
R
NA-induced silencing is a process which allows cells to
regulate the synthesis of specific proteins. RNA silencing
is promoted by the protein C3PO (component 3 of RISC).
We have previously found that phospholipase Cβ, which
increases intracellular calcium levels in response to specific G
protein signals, inhibits C3PO activity towards certain genes.
Understanding the parameters that control C3PO activity and
which genes are impacted by G protein activation would help
predict, which genes are more vulnerable to down-regulation?
Here, using a library of 1018 oligonucleotides, we show that
C3PO binds oligonucleotides with structural specificity but
little sequence specificity. Alternately, the rate of hydrolysis is
exquisitely sensitive to the substrate stability. Importantly, we
find that oligonucleotides with higher Tm values are inhibited
by bound PLCβ. This finding is supported by microarray analysis
in cells over-expressing PLCβ1. Taken together our work enables
predictions of the genes whose post-transcriptional regulation
is responsive to the G protein/phospholipase Cβ/calcium
signaling pathway.
Speaker Biography
Suzanne Scarlata is a Professor Emeritus of Stony Brook University and a Whitcomb
Chair at Worcester Polytechnic Institute. Most of her research has focused on the
regulation of G protein signaling in model systems and in cultured cells using primarily
fluorescence methods. The work presented here represents an unexpected connection
between the impact of extrasensory information and post-transcriptional gene
regulation through the Gαq/phospholipase Cβ signaling pathway.
e:
sfscarlata@wpi.edu