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academies
J Pharmacol Ther Res 2017 Volume 1 Issue 2
November 02-03, 2017 Chicago, USA
4
th
International Congress on
International Conference and Exhibition on
Drug Discovery, Designing and Development
Biochemistry, Molecular Biology: R&D
&
Nanostructured Lipid Carriers (NLC)-Based gel for the topical delivery of azelaic acid: Designing,
characterization and
in-vitro
evaluation
Deepinder Singh Malik
and
Gurpreet Kaur
Punjab University, India
A
zelaic acid (AZA) is a naturally occurring dicarboxylic
acid, reported to be effective in management of mild to
moderate acne vulgaris. However, few noticeable dose-related
side effects limit its therapeutic applicability. Therefore, the
study was directed towards the optimization, formulation
and evaluation of the AZA loaded nano-structured lipid carrier
(NLCs) to enhance its payloads and achieve sustained release
at the target site. NLCs were prepared by melt emulsification
and ultra-sonication method employing glyceryl monostearate
and oleic acid as solid and liquid lipid, respectively. The
formulation was optimized employing design expert software
taking sonication time, amplitude and drug concentration as
independent variableswithparticle sizeanddrug entrapment as
dependent variables. The optimized preparation so formed was
incorporated intoaloe-verabasedcarbopol gel andevaluatedfor
its size, morphology, pharmacokinetic and pharmacodynamic
parameters. NLCs were found to possess mean particle size in
a range of 45-48 nm with low polydispersity index value (~ 0.4)
and encapsulation efficiency of ca. 82%. It was further verified
employing transmission electron microscopy which depicted
the formation of uniform surfaced spherical nanoparticles.
In-vitro
permeation and skin retention studies revealed
significant retention of AZA within the skin with minimum
penetration across the skin. Draize patch test exhibited no
signs of irritation/lesion on the skin indicating its non-irritating
nature. Skin distribution analysis employing rhodamine 6G as
a fluorescent dye unveiled the deposition of NLCs preparation
to the deeper layers of skin. Thus, as per experimental findings,
NLCs may be explored as promising carriers for site specific
targeting.
e:
deepinder.malik88@gmail.com