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J Pharmacol Ther Res 2017 Volume 1 Issue 2
November 02-03, 2017 Chicago, USA
4
th
International Congress on
International Conference and Exhibition on
Drug Discovery, Designing and Development
Biochemistry, Molecular Biology: R&D
&
Stability indicating UHPLC method for the assay of maraviroc in bulk and in formulations
Geetha Reddy M
Dr. Reddy’s Laboratories, India
A
n Ultra High Pressure Liquid Chromatographic method
was developed for the estimation of Maraviroc in
bulk and in formulations. The separation was achieved
on X bridge (C18 20 x 4.6 mm, 2.5 μ column) using 0.01M
potassium dihydrogen phosphate (KH2PO4) (pH 7.0 adjusted
with ortho phosphoric acid) and acetonitrile (60:40) as
mobile phase. The flow rate kept at 0.5 mL/min, column
temperature 30°C, and the column eluents were monitored
at 210 nm. The forced degradation studies were done to
show stability indicating power of the method. The method
has been validated accordance with ICH guidelines for
specificity, precision, accuracy, linearity, limit of detection,
limit of quantification, robustness and ruggedness. The
results were found to be well within the limits. The method
can be used for the routine analysis of Maraviroc bulk and in
formulations.
e:
geetharm@drreddys.com