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J Pharmacol Ther Res 2017 Volume 1 Issue 2
November 02-03, 2017 Chicago, USA
4 th International Congress on
International Conference and Exhibition on
Drug Discovery, Designing and Development
Biochemistry, Molecular Biology: R&D
&
The aim of the research was to study the influence of the
multiprobiotic “Symbiter forte omega” on pathological
changes in soft tissues of rats’ periodontal tissues under the
conditions of prolonged use of omeprazole. Experiments
were performed on 46 white male rats weighing 180-250 g.
The animals were divided into 3 groups: I - control, 0.2 ml
of water for injection intraperitoneally was administered to
rats daily for 28 days; ІІ - rats that received omeprazole daily
(during 28 days) (14 mg / kg body weight intraperitoneally);
III - omeprazole (14 mg / kg body weight intraperitoneally) in
combination with “Symbiter forte omega” (0.14 ml / kg body
weight orally) was administered. Animals were sacrificed
under urethane anesthesia (50 mg / kg body weight
intraperitoneally) by bloodletting. The object of the study
was soft periodontal tissues, in which the content of free
oxyproline (Tetyanets S.S., 1985), fucose (Sharaev PN, 1997)
and glycosaminoglycans (GAG) (Sharaev PN, 1987 ) was
determined. The content of oxyproline in soft periodontal
tissues after 28-day omeprazole administration increased in
1.87 times (P <0.05) when compared with control. Content
of oxyproline in soft periodontal tissues of rats which
recieved multiprobiotic “Symbiter forte omega” showed a
decrease in comparison with animals without correction in
1.49 times (P <0.05). We estimated that the content of GAG
in soft periodontal tissues of rats under conditions of long
hypoacidity on 28 day of the administration of omeprazole
increased in 1.37 times (P <0.05) when compared to control
group. The use of the multiprobiotic “Symbiter forte omega”
during 28 days on the background of omeprazole-induced
hypoacidity contributed to reduction in GAG content in
1.89 times (P <0.05) in soft periodontal tissues of rats when
compared with non-corrected animal group. Investigating
the content of free fucose in soft periodontal tissues under
conditions of omeprazole-induced hypoacidity, the following
results were obtained: the multiprobiotic “Symbiter forte
omega” reduced the content of fucose in periodontal
tissues in 1.61 times (P<0.05) in comparison with the control
group and in 1.12 times (P <0.05) when compared to the
rats without correction. Thus, in conditions of prolonged
omeprazole-induced hypoacidity, there is an increased
catabolism of collagen and non-collagen proteins in rats’
periodontal tissues. Multiprobiotic “Symbiter forte omega”
protects against depolymerization of collagen and non-
collagen structures of periodontal connective tissue under
conditions of long hypoacidity.
e:
Influence of multiprobiotic on concentration of Collagen and Non-Collagen protein monomers in rat’s
parodont during continuous hypoacidosis
A.O. Mykytenko, K.S. Neporada, T.V. Berehova and D.S. Yankovsky
Ukrainian medical stomatological academy, Ukraine