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J Pharmacol Ther Res 2017 Volume 1 Issue 2

November 02-03, 2017 Chicago, USA

International Congress on

International Conference and Exhibition on

Drug Discovery, Designing and Development

Biochemistry, Molecular Biology: R&D

artially or fully sub-1 µm and sub-2 µm porous silica

particles have achieved more interests as column

packing materials in separation due to enhanced separation

efficiency, fast separation and high separation resolution.

Sub-1 µmporous silicamonolith particles have been prepared

successfully prepared by sol-gel process followed by grinding

and calcinations at 550. A high-efficient HPLC stationary

phase based on porous silica monolith particles has been

prepared by reacting 4-chloromehtylphenylisocynate (4-

CPI) to porous partially sub-1 µm monolithic silica particles

via isocyanate-hydroxyl reaction using dibutyltin dichloride

(DBTDC) as a catalyst followed by initiator attachment and

RAFT polymerization of styrene. The resultant phase was

packed in glass lined stainless steel micro-column (1.0 mm

x 300 mm), and the separation efficiencies as high as 60,000

plates (200,000/meter) were achieved for the separation

of peptides and proteins using 60/40 acetonitrile/50 mM

ammonium format (v/v %) with at a flow rate of 25 µL/min.

The separation efficiency of this new phase is comparable

or even better than some of commercial available stationary

phases. This phase has shown some encouraging possibility

for fast analysis when packed in a short column. This study

offers a promising vision towards commercialization of

chromatographic phases based on silica monolith particles.

Separation of synthetic peptides and proteins by polystyrene bound silica monolith particles as HPLC

stationary phase

Ashraf Ali

and

Won Jo Cheong

Inha University, South Korea