27 / 27
Page 83
allied
academies
J Pharmacol Ther Res 2017 Volume 1 Issue 2
November 02-03, 2017 Chicago, USA
4
th
International Congress on
International Conference and Exhibition on
Drug Discovery, Designing and Development
Biochemistry, Molecular Biology: R&D
&
O
ne of the most harmful and destructive disease of
papaya is the papaya ringspot (PRS) disease caused
due to the PRSV (
Papaya ringspot virus
). It belongs to the
family
Potyviridae
and genus
Potyvirus
. PRSV virions are
filamentous, flexuous in nature, approximately 780 nm x 2
nm long and it contains a single molecule of linear positive
sense ssRNA approximately 10.3 kb in length. The entire virus
consists of the following genes:
P1, HC-Pro, P3, 6K1, CI, 6K2,
VPg, NIa, NIb
and
CP
. These genes were cloned into pGEMT-
easy vector and sub cloned into binary vector pCB302.
After that agroinfiltration and on-spot silencing assay of
the individual genes were done using UV Fluorescence
Spectrophotometer to check the silencing suppression
characteristics of individual genes. An emission spectrum
was obtained using UV Fluorescence Spectrophotometer
to further confirm the obtained results. In UV Fluorescence
Spectrophotometer the excitation wavelength was set as
309 nm and the emission wavelength was set as 509 nm.
From the spectrum obtained it can be presumed that CI and
HC-Pro Hyd are potent silencing suppressors of PRSV-Hyd.
The main objective of this project was characterization of
PRSV (
Papaya ringspot virus
) encoded proteins for silencing
suppression characteristics.
e:
ldebosmita@gmail.comCharacterization of
papaya ring spot virus
(PRSV) genome encoded proteins for silencing suppression
characteristics
Debosmita Lahiri
1
and
Kodetham Gopinath
2
1
VIT University, India
2
University of Hyderabad, India