13 / 27
Page 69
allied
academies
J Pharmacol Ther Res 2017 Volume 1 Issue 2
November 02-03, 2017 Chicago, USA
4
th
International Congress on
International Conference and Exhibition on
Drug Discovery, Designing and Development
Biochemistry, Molecular Biology: R&D
&
T
he ethanolic extract of
Alafia barteri
(Hook F. Icon) was
dissolved in distilled water and successively partitioned
in n-hexane, chloroform, ethyl acetate and n-butanol. The
fractions were evaluated for phytochemical composition,
lethality against brine shrimp larvae and
in-vitro
antiplasmodial
activity against
Plasmodium falciparum
strain. The obtained
results revealed that the roots and leaf extracts of
A. barteri
exhibited broad spectrum of antiplasmodial activity (IC50
1.5±0.7 - 6.2±0.80 µg/mL). The aqueous leaf fractions
displayed the most potent antiplasmodial activity with
an IC50 value of 1.5±0.7 µg/mL, which is comparable to
reference antimalarial drug (IC
50
value of 1.3±0.2 µg/mL). The
leaf fractions displayed higher activity than the root extracts.
The highest minimum lethal concentration (105.2±0.8 ppm)
was exhibited by the aqueous leaf extract followed closely by
the root extract (120.2±1.1 ppm). The leaf extracts contained
higher polyphenols (45.3±0.85 mgGAE/g) and flavonoids
(18.10±0.2 mgCTE/g) than the root extracts. The n-hexane
and EtOAc extracts/fractions displayed lower activity on
brine shrimp larvae.
e:
lasialiyu@yahoo.comPhytochemical composition, cytotoxicity and
in-vitro
antiplasmodial activity of fractions from
Alafiabarteri
olive (Hook F. Icon)-
Apocynaceae
Lasisi A A
1
, Olayiwola M A
2
, Balogun S A
1
, Akinloye O A
1
and
Ojo D O
1
1
Federal University of Agriculture, Nigeria
2
Federal College of Education, Nigeria