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Journal of Biotechnology and Phytochemistry
Volume 1 Issue 3
Chemistry World 2017
Page 78
November 13-15, 2017 Athens, Greece
7
th
World Congress on
Chemistry
J Biotech and Phyto 2017
Sizing α, β and γ cyclodextrins by capillary
electrophoresis and indirect UV detection
Yin Zhou
The University of British Columbia, Canada
Cyclodextrins have ring structures made of glucose
connected by 1,4-glycoside linkage and they differ in the
number of glucose on the ring. The interior of cyclodextrin is
considerably less hydrophilic than its exterior; therefore, they
are useful for carrying hydrophobic molecules. Due to their
hydrophilic exterior, they are able to penetrate body tissues,
which make them good candidates as drug carriers where
they can release biologically active compounds under specific
conditions. However, different sizes of cyclodextrins form
different complex with the same molecule, so, it is important
to know the size of each cyclodextrin. Indirect UV-capillary
electrophoresis and Taylor dispersion analysis are used to size
α, β and γ cyclodextrins. Because cyclodextrins have low UV
absorbance, indirect UV is used in which the background
electrolyte has significantly higher UV absorbance than the
target molecules, therefore, resulting in negative peaks. Using
TDA, diameters of α- and γ-CD are calculated to be 0.70
nm and 0.86 nm. The small standard deviation indicated the
precise and reproducible measurement.
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