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Journal of Biotechnology and Phytochemistry
Volume 1 Issue 3
Chemistry World 2017
Page 77
November 13-15, 2017 Athens, Greece
7
th
World Congress on
Chemistry
A novel double-layer immobilised biocatalyst
for simulated barrel aging production of sweet
wine
Vassilios Ganatsios
and
Spiridon Mammalis
Eastern Macedonia and Thrace Institute of Technology, Greece
The present study was undertaken to assess the simulating
barrel aging effects of a novel double-layer immobilised
biocatalyst for low temperature wine making. The double-
layer biocatalyst consisted of partially delignified and burned
oak sawdust (DBOS) with entrapped
Leuconostoc oenos
cells,
covered with starch gel containing the alcohol resistant and
cryotolerant strain
Saccharomyces cerevisiae
AXAZ-1. The
immobilized double-layer biocatalyst was found efficient for
high gravity grape must fermentations at low temperature
and high final alcohol concentration. In parallel, this novel
biocatalyst was able to convert malic acid to lactic acid and
simulating oak barrel ageing through extraction of volatiles
of burned oak sawdust producing a sweet wine with higher
organoliptic characteristics as shown by GC and SPME GC/
MS analysis. Improvement of wine quality compared with
wine fermented with free
S. cerevisiae
cells was attributed
to malolactic fermentation and lower alcohols production
due to the low fermentation temperature. The significance
of DBOS is the feasibility of three processes (alcoholic
fermentation, malolactic fermentation, oak barrel aging
simulation processes) in one batch fermentation reducing
the production cost and energy demand in the food industry,
giving the opportunity to the consumers to taste an aromatic
sweet wine without preservatives.
v.gkanatsios@gmail.comJ Biotech and Phyto 2017