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Asian Journal of Biomedical and Pharmaceutical Sciences | ISSN: 2249-622X | Volume 8
&
Joint Event
Chemistry and Organic Chemistry
Biomedicine & Pharmacotherapy
International Conference on
8
th
World Congress on
October 22-23, 2018 | Frankfurt, Germany
Role of the kupffer cell cd68, a plasmodium sporozoite receptor, in modulation of experimental
cerebral malaria (ECM)
Sung-Jae Cha
Johns Hopkins Bloomberg School of Public Health, USA
M
alaria infection of a vertebrate host starts with liver
infection by Plasmodium sporozoites. Sporozoites
move from the mosquito bite site to the liver via the blood
circulation and leave the circulation by traversing Kupffer cells
that line the liver blood vessels. Traversal requires interaction
between the CD68 Kupffer cell receptor and the sporozoite
surface-GAPDH ligand. We previously reported that a strong
( ~ 70 %) reduction occurs in the efficiency of sporozoite liver
invasion in CD68 knockout (KO) mice compared to wild-
type controls. We made the unexpected observation that
the development of experimental cerebral malaria (ECM)
in these CD68 KO mice is strongly inhibited. This inhibition
only occurs when the mice are infected with sporozoites,
not when infected with blood-stage parasites. Importantly,
transfer of plasma from a sporozoite-infected CD68 KO
mouse into a wild-type mouse induces the ECM-inhibitory
phenotype in the recipient mouse. Our initial experiments
found the plasma from sporozoite-infected CD68 KO mice
has a dramatically different biomarker activation profile
compared to wild-type (WT) mice. We hypothesize that
sporozoites traverse Kupffer cells or endothelial cells by
breaching them and causing cellular injury in the absence
of a CD68 receptor. We have identified soluble plasma
factor(s) that are responsible for ECM inhibition in the
sporozoite-infected CD68 KO mice and are determining the
factors that promote their synthesis. The results may lead
to novel approaches for the prevention of cerebral malaria
development and malaria death.
Speaker Biography
Sung-Jae Cha have over 20 years’ experience as a research scientist in biological
science field covering cell and molecular biology, genetics, immunology and molecular
parasitology. His recent research has focused on the molecular biology of malaria
parasite-mammalian liver cell interactions. Using phage display library screening
technique he has identified the Kupffer cell CD68 and the Plasmodium surface GAPDH
as a receptor and a ligand for malaria sporozoite liver invasion respectively.
e:
scha5@jhu.eduSung-Jae Cha, Chemistry and Biomedicine 2018, Volume 8
DOI: 10.4066/2249-622X-C4-012