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academies
Cell Science, Stem Cell Research &
Pharmacological Regenerative Medicine
November 29-30, 2017 | Atlanta, USA
Annual Congress on
Adv cel sci tissue cul 2017 | Volume 1 Issue 2
Co-regulation of the Glycine max soluble N-ethylmaleimide-sensitive fusion protein attachment
protein receptor (SNARE)-containing regulation occurs during defense to a root pathogen
Keshav Sharma
Mississippi State University, USA
H
eterodera glycines, also known as Soybean Cyst
Nematode (SCN) is a major pathogen of soybean (Glycine
max), causes nearly a billion-dollar loss in U.S. every year
(Wrather et al. 2001; Wrather and Koenning, 2006; Smolik
and Draper, 2007; Koenning and Wrather, 2010). Efforts
to combat SCN include production of resistant soybean
varieties, use of nematicides, improved crop rotation and
bio-control methods (Wrather et al. 1984; Chang et al. 2011).
However, effective control has not been achieved yet. Study
of host plant interactions at the cellular level is important
as it may provide new species-specific means of controlling
SCN (Klink et al. 2007). We are conducting various molecular
approaches to find actual cellular mechanism of host
resistance. Closer study of infected cells in resistant variety
G. max [Peking/PI548402] and susceptible variety G. max
[Williams 82(PI518671)] through laser microdissection have
resulted various unique genes present in G. max [Peking/
PI548402] (Klink et al. 2007; 2009). Overexpression of these
genes in susceptible cultivar G. max [Williams 82(PI518671)]
have resulted resistance by inducing incompatible reaction
and RNA interference of these genes in resistant genotypes
resulted susceptible reaction, thereby inducing compatible
reaction (Matsye et al. 2012; Pant et al. 2014). In this
approach we have overexpressed the components of the
Soluble N-ethylmaleimide-sensitive fusion (NSF) Attachment
Protein (SNAP) REceptor (SNARE) complex that helps in
docking of the vesicles to the membrane and subsequent
release of the vesicular contents to the apoplast (Jahn and
Fasshauer et al. 2012; Matsye et al. 2012; Pant et al. 2014).
There are many proteins that play significant role in this
process however, the core components of this study are
syntaxin 121 (SYP121), Synaptosomal-associated protein
25 (SNAP-25), Synaptotagmin (SYT), Synaptobrevin (SYB),
Secretion 1/mammalian uncoordinated-18 ([Sec1]/Munc18)
and N-ethylmaleimide-sensitive fusion protein (NSF).
Syntaxin 121, G. max homolog of Saccharomyces cerevisiae,
Suppressor of sec1 (SSO1) known as PENETRATION1 (PEN1)
in Arabidopsis thaliana, (Collins et al. 2003) function in
resistance to Heterodera glycine. Co-expression of SYP121
with SNARE homologs results elevated transcripts in infected
cells inducing resistance reaction.
e:
ks1697@msstate.edu