cell-science-pharmacology-2017-posters-abstracts

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allied

academies

Cell Science, Stem Cell Research &

Pharmacological Regenerative Medicine

November 29-30, 2017 | Atlanta, USA

Annual Congress on

Adv cel sci tissue cul 2017 | Volume 1 Issue 2

Sendai virus recruits cellular villin to remodel actin cytoskeleton during fusion with hepatocytes

Sunandini Chandra

University of Delhi, India

econstituted Sendai viral envelopes (Virosomes) are well

recognized for their promising potential in membrane

fusion mediated delivery of bioactive molecules to liver cells.

Despite the known function of viral envelope glycoproteins

in catalyzing fusion with cellular membrane, the role of

host cell proteins remains elusive. Here, we used two-

dimensional differential in-gel electrophoresis (2D-DIGE)

to analyze hepatic cells in early response to virosome-

induced membrane fusion. Quantitative mass spectrometry

together with biochemical analysis revealed that villin,

an actin-modifying protein, is differentially up-regulated

and phosphorylated at Threonine-206 (T206), as an early

molecular event during membrane fusion. We found that

villin influences actin dynamics which, in turn, promotes

membrane mixing through active participation of Sendai

viral envelope glycoproteins. Modulation of villin in host

cells also resulted in a discernible effect on the entry and

egress of progeny Sendai virus. Taken together, these results

suggest a novel mechanism of regulated viral entry in animal

cells mediated by host factor villin.

Speaker Biography

Sunandini Chandra is trained in the field of virus-host interactions, especially in the field

of viral fusion with host cell membrane. After a Master’s in Biotechnology, she recently

completed her Doctoral research work in Sendai virus-host cell interactions, with

special emphasis on the role of actin modifying proteins in fusion. Her work employed

proteomic approaches and is the first to result in identifying a host cell protein- villin,

implicated in virus induced membrane fusion with the host cell membrane. This

work provides an insight into the mechanism of membrane fusion mediated entry

of enveloped viruses and may be exploited for therapeutic interventions for other

related pathogenic viruses. Also, this information could be exploited to improve fusion

efficiency of Sendai virosomes, for efficient targeted delivery to liver cells.