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Biotechnology Congress 2018 & Emerging Materials 2018
Biomedical Research
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ISSN: 0976-1683
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Volume 29
S e p t e m b e r 0 6 - 0 7 , 2 0 1 8 | B a n g k o k , T h a i l a n d
allied
academies
Joint Event on
EMERGING MATERIALS AND NANOTECHNOLOGY
BIOTECHNOLOGY
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Annual Congress on
Global Congress on
Indu Thakur, Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C4-011
RADIATION DOSE RESPONSE AND DOSE
MODIFICATION FACTOR OF
PHYLLANTHUS
NIRURI
ON SWISS ALBINO MICE
STANDARDIZATION OF RADIOPROTECTIVE
EFFECT
Indu Thakur
Barkatullaha University, India
T
his study was aimed to assess the optimum route administration
for
Phyllanthus niruri
(family - Euphorbiaceae) alcoholic extract with
maximum radioprotection by evaluating dose modification factor (DMF)
and dose response on mice against different strengths of irradiation to
establish the dosage profile. To optimize route of administration
P. niruri
alcoholic extract was administered via intraperitoneal (i.p), intramuscular
(i.m), intravenous (i.v) and oral route 1 hr before 4 Gy irradiation and after
24 hr the percentage of aberrant cells were calculated. DMF was calculated
by observing survival rate following whole body irradiated with 8, 9, 10 and
11 Gy radiation exposure with and without 200 mg/kg, i.p,
P. niruri
alcoholic
extract before 1 hr of exposure. Radiation dose response effect of 200
mg/kg of
P. niruri
alcoholic extract was observed against 1, 2, 3 and 4 Gy
gamma ray exposure by scoring different types of chromosomal aberrations
from bone marrow metaphase plates. The i.p administered group showed
significantly reduced aberrant cell percentage compared to i.m, oral, i.v and
sham control group. DMF was calculated to be 1.12 as evaluated by radiation
LD50 determination with and without
P. niruri
alcoholic extract at 200 mg/
kg dose.
P. niruri
alcoholic extract significantly (P<0.05-0.001) reduced
percent aberrant cell and major aberrations like breaks, rings and polyploidy
against 4 Gy radiation. The dose of 200 mg/kg was found to have maximum
radioprotective potential in i.p route. It showed DMF of 1.12 with improved
survival rate, delayed occurrence of lethality and radiation sickness. Alcoholic
extract of
P. niruri
decrease the complex aberrations like rings, dicentrics and
SDC indicating significant protection of bone marrow against double strand
breaks and the multiple lesions in chromosomes. The optimum dose of
P.
niruri
alcoholic extract is established to be 200 mg/kg, i.p., having safe and
effective radioprotector efficiency.
Indu Thakur, Biologist, Scientist, Educator completed her
BSc from Barkatullaha University, Bhopal and MSc in
Botany specialization from Biotechnology. She received
her PhD. Degree in Life Sciences from, Barkatullaha Uni-
versity Bhopal under Senior Research Fellowship award-
ed by Indian Council of Medical Research New Delhi. She
also recieved Junior research fellowship from Defense
Reseaearch Development Organisation, New Delhi for 2
years. She served as a faculty in Indore Public School,
Indore,and aslo worked as clinical hypnotherapist cer-
tified by califorinia University, New Delhi. Her present
research projects are focused on phytopharmacological
work on active isolated constituents from Indian folklore
medicine directed to explore their therapeutic potential
and attempting on formulation of standardized product
by following the modern herbal Ayurvedic monographs
and international guidelines. Her field of research fo-
cuses on pharmacological screening, pharmacokinetic,
pharmacodynamic and bioavailability studies, drug-food
interactions, and standardization method development
for herbals and chromosomal aberrations study on an-
imal model.
Itjnchrc38@gmail.comBIOGRAPHY