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Note:
Biotechnology Congress 2018 & Emerging Materials 2018
Biomedical Research
|
ISSN: 0976-1683
|
Volume 29
S e p t e m b e r 0 6 - 0 7 , 2 0 1 8 | B a n g k o k , T h a i l a n d
allied
academies
Joint Event on
EMERGING MATERIALS AND NANOTECHNOLOGY
BIOTECHNOLOGY
&
Annual Congress on
Global Congress on
Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C4-011
SOME IMPORTANT CONCEPTS
FOR LEAF PROTEIN EXTRACTION
AND TWO-DIMENSIONAL
ELECTROPHORESIS TECHNIQUE
Daryush Talei
Shahed University, Iran
P
roteomic analysis of plants relies on high yields of pure
protein. In plants, protein extraction and purification
to perform two-dimensional electrophoresis technique
present a great challenge due to accumulation of a large
amount of interfering substances including polysaccharides,
polyphenols and secondary metabolites. Therefore, it is
necessary to modify and use an effective protein extraction
protocols for two-dimensional electrophoresis technique.
To achieve high yields of pure protein and successful two-
dimensional electrophoresis some important factors during
protein extraction process, protein precipitation methods, iso-
electric focusing program such as; rehydration types (passive
or active), isoelectric point (PI), IPG strips (size and pH),
power conditions and resolution in IEF, equilibration buffer,
SDS-PAGE process such as; choosing a gel size format, gels
percentage and power conditions, detection of protein in
gels, image achievement and analysis, and identification and
characterization of 2-D protein spots should be considered.
SYNTHESIS, PROPERTIES AND
APPLICATIONS OF NOBLE METAL
NANOPARTICLE-BIOMOLECULE
INTEGRATION
Xianfang Zhu
Xiamen University, China
N
oble metal nanoparticles, such as gold or silver
nanoparticles and nanorods exhibit unique photonic
and electronic properties. Functionalization of noble metal
nanoparticles with biomolecules (e.g., protein and DNA) has
numerous applications in catalysis, delivery, therapy, imaging,
sensing, constructing nanostructure and controlling structure
of biomolecules. In this paper, we review recent research
about noble metal nanoparticle-biomolecule integration
from the following three aspects: synthesis of noble metal
nanoparticle-biomolecule integration, including electrostatic
adsorption, direct chemisorption of thiol derivation, covalent
binding through bifunctional linkers and specific affinity
interaction; photonic properties and bioactivation of the nobel
metal nanoparticle-biomolecule conjugation; applications in
biosensor, imaging, diagnosis and therapy in medicine, and
assembly of nanoparticle. The special attention has been
paid on the conjugation of noble metal nanoparticle and
biomolecule as well as the most recent related applications.