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allied
academies
Joint Event
February 21-22, 2019 | Paris, France
Microbiology & Applied
Microbiology
2
nd
International Conference on
World Congress on
Wound Care, Tissue Repair
and Regenerative Medicine
&
Journal of Trauma and Critical Care | Volume 3
A Study on prevalence of
Escherichia Coli
O157 in healthy camels, cattle, sheep and goat from
slaughterhouse in Al Ain, the United Arab Emirates
Dawood Sulaiman Al Ajmi, Sharmila Banu
and
Shafeeq Rahman
UAE University, UAE
S
higa toxin-producing
Escherichia coli
(STEC) are
E. coli
strains,
whichareassociatedwithmajorfoodillnessaroundtheworld.
E. coli
O157has beenwidely reportedas themost commonSTEC
serogroup, which have emerged as important enteric pathogen
since their identification. These bacterial strains are colonized
in animals and are excreted through animal feces, which can
contaminate the farm, water and foodprocessing environment.
This study aims to evaluate the prevalence of
E. coli
serotype
O157 in feces of cattle, camel, sheep and goat slaughtered
in UAE for meat consumption. This study was carried out on
fecal samples of healthy cattle (n=137), camel (n=140), sheep
(n=141) and goat (n=150) during the period of September 2017
to August 2018. It was found that
E. coli
O157 was present in
the fecal samples of goat, camel and cattle at 2%, 3.3%, and
16% respectively. Surprisingly, from the samples collected from
sheep we failed to detect any
E. coli
O157 strains. We have
used the traditional sensitive Immuno-magnetic separation
technique (IMS) coupled with culture plating method for
detecting the
E. coli
O157 pathogen. All isolates were confirmed
as
E. coli
O157 using latex agglutination test (Oxoid) and the
virulent genes were confirmed using multiplex PCR. These
results comprise the first report on
E. coli
O157 prevalence
in the UAE and prove the presence of these pathogens in
the slaughtering animals, which could possibly contaminate
the meat products. This study also indicated that there were
no breed and seasonal effect on these strains in the UAE.
Speaker Biography
Dawood Sulaiman Al Ajmi has completed his PhD in animal production from the University
of Queensland in 2008. He is currently working as an assistant professor in college of food
and agriculture at arid land agriculture department, UAE University. His main area of
research is in veterinary science and have publications in reputed journals.
e:
dawoods@uaeu.ac.aeDawood Sulaiman Al Ajmi et al.
, J Trauma Crit Care, Volume 3
DOI: 10.4066/2591-7358-C1-003