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Joint Event
February 21-22, 2019 | Paris, France
Microbiology & Applied
Microbiology
2
nd
International Conference on
World Congress on
Wound Care, Tissue Repair
and Regenerative Medicine
&
Journal of Trauma and Critical Care | Volume 3
Isolation and characterization of phenol-degrading yeasts from industrial effluent (Petrochemical
Seaport Mahshahr, Iran)
Atena Alirezaei Dizicheh
1
, Mohammad Ghayyomi Jazeh
2
, Mansour Bayat
1
and
Mahmood Alimohmmadi
2
1
Islamic Azad University, Iran
2
Tehran University of Medical Science, Iran
N
owadays environmental pollutants are one of the problems
facing the industrial world. Among these compounds,
phenolic compounds are toxic pollutants to which belongs
chlorophenols known as xenobiotic chemicals. 4-Chlorophenol
(4-CP) is one of the chlorophenols with a high solubility inwater,
so it is most detected in wastewater and also can accumulate in
their bodies biologically.
In present study 13 strains of bacteria and 6 strains of yeast and
mold phenol degradationwas purified fromShahid Tondgooyan
Petrochemical wastewater treatment unit was first carried
out within about 15 days. Then, capability of the isolated
microorganisms in biodegradation of 100ppm 4-chlorophenol
in presence of 2 g/l glucose as a growth substrate was
examined. Two microorganisms, selected as superior species.
The strains were designated TY1 and TY2 and Strains were
identified by molecular method using amplification of ITS
gene region. The phenol degradation was determined by the
spectrophotometric method 4-amino antipyrine. The results
showed that 100% removal of 100ppm 4-Chlorophenol by TY1
in 45 hrs, TY2 in 21 hrs and mixed culture of TY1TY2:50/50 in
presence of 2 gr/l glucose within 18 hrs. Percentage of pure
cultures in mixed culture had no significant effect on 4-CP
removal efficiency. Furthermore, the results of the sequencing
showed that the isolates with the genus
Trichosporon sp
. The
significance and impact of the study is the utilization of native
yeast strains isolated fromthewastewater itself havingpotential
for environmental bioremediation in petroleum refinery and
petrochemical industries.
Speaker Biography
Atena Alirezaei Dizicheh is an Iranian university teacher at Rasht, in the north of Iran. She
was graduated in the Foundry field at Tehran University and earnedmaster degree in 2005.
She began to work as research and development unit Manager at Gilan Steel Complex
in 2006 and simultaneously taught corrosion in the building, steel project, principles of
building maintenance in University of Applied Science and Technology of Rasht Academic
Center for Education. In recent fourteen years, she has taught many courses in Karaj
House of Worker University of Applied Science and Technology, Rasht Academic Center for
Education and Mouj Nonprofit University. She has codified 5 single course and one single
module in University of Applied Science and Technology that is in the stage of approval.
e:
Atena.Alirezaei@gmail.comAtena Alirezaei Dizicheh et al.
, J Trauma Crit Care, Volume 3
DOI: 10.4066/2591-7358-C1-003