10 / 33
Page 42
Notes:
allied
academies
Joint Event
February 21-22, 2019 | Paris, France
Microbiology & Applied
Microbiology
2
nd
International Conference on
World Congress on
Wound Care, Tissue Repair
and Regenerative Medicine
&
Journal of Trauma and Critical Care | Volume 3
Immobilization and application of laccase preparation
Vladimir Elisashvili
Agricultural University of Georgia, Georgia
T
he application of the white rot basidiomycetes (WRB)
and especially their lignin-modifying enzymes in various
industrial biotechnologies and in bioremediation of wastewater
and soil polluted with the emerging organic pollutants (EOP)
requires their huge production and stabilization. In this
study, production of laccase by two most promising enzyme
producers (
Trametes versicolor
and
Cerrena unicolor
), isolated
and identified from the Georgian forest, was optimized in
submerged fermentation conditions and laccase preparations
were isolated from the culture liquids by ammonium sulfate
precipitation. Two obtained laccase preparations and, for
comparison, commercial laccase from
Trametes versicolor
were immobilized on the fumed silica nanoparticles (fsNP)
with APTES modification in order to increase their stability
and to provide their separation from the reaction mixture and
reusability. The coupling procedure for fsNP–laccase conjugates
was optimized to bind asmuch as possible of the initially applied
laccase and to obtain the highest loads of laccase activity on
the fsNP. Among the enzyme preparations, immobilization of T.
versicolor laccase was the most effective - 4.0 U g−1 fsNP, with
immobilization yield of 121% and the washing loss of 3.1%.
Immobilization yield of
C. unicolor
laccase achieved 133% with
the least washing loss - 0.9%; however, enzyme load was only
2 U g-1. Finally, the immobilization yield for commercial laccase
preparation was the highest (172%), but the washing loss was
3.2% and enzyme load was 2.5 U g-1. We have tested laccase
preparations for degradation of micropollutants of waste-
water. There was shown that immobilized laccase preparations
appeared to be appropriate tools for elimination of diclofenac
and Bisphelol A.
Speaker Biography
Vladimir Elisashvili is a director of the Animal Husbandry and Feed Production Institute
of the Agricultural University of Georgia. In 1974, he obtained his PhD in microbiology
at the Saint Petersburg (Leningrad) State University. Then, he continued his work at
the chair of microbiology as a scientist and assistant professor. In 1983, he joined
the Durmishidze Institute of Biochemistry and Biotechnology, Tbilisi, Georgia and
in 1993, he obtained the Doctor of Biological Sciences degree in biochemistry. In
1976/77, he obtained a postdoc position at the Institute of Molecular Biology, Paris
VII, France. In 2001-2006, he worked at the Free University of Brussels and in two
biotechnological companies in Israel. He has over 190 publications and supervised 21
PhD theses. He is serving as an editorial board member of three reputed Journals.
e:
v.elisashvili@agruni.edu.geVladimir Elisashvili
, J Trauma Crit Care, Volume 3
DOI: 10.4066/2591-7358-C1-003