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J Parasit Dis Diagn Ther 2017
Volume 2 Issue 3
Tropical Medicine 2017
Page 53
September 7-8, 2017 | Edinburgh, Scotland
4
th
International Conference on
Tropical Medicine, Infectious Diseases & Public Health
DYNAMICS OF TRANSGENIC ENTERO-
BACTER CLOACAE-GFP-DEFENSIN IN
ANOPHELES STEPHENSI UNDER LABORA-
TORY CONDITION
Mohammad Ali Oshaghi
a
, Hossein Dehghan
a
, Mohammad Reza Abai
a
,
Fatemeh Mohtarami
a
,Hassan Vatandoost
a
, Seyed Hassan Moosa-Kazemi
a
,
Bagher Yakhchali
b
, Naseh Maleki-Ravasan
c
, Habib Mohammadzadeh
d
,
a
Tehran University of Medical Sciences, Iran
b
National Institute of Genetic Engineering and Biotechnology, (NIGEB), Iran
c
Pasteur Institute of Iran, Iran
d
Urmia University of Medical Sciences, Iran
Background:
Enterobacter cloacae bacterium is a known
symbiont of most Anopheles gut microflora and nominated
as a good candidate for paratransgenic control of malaria.
However, the population dynamics of this bacterium within
An. stephensi and its introduction methods to the mosquitoes
have not yet been explored.
Methods:
Enterobacter cloacae subsp. dissolvens expressing
green fluorescent protein-defensin (GFP-D) was used to
study transstadial transmission and the course of time, larval
habitat, sugar, and blood meal on dynamics of the bacterium
in the mosquito life stages. The bacterial quantities were
measured by plating samples and counting GFP expressing
colonies on the Tet-BHI agar medium
Results:
The E. cloacae population remained stable in sugar
bait at least for eleven days whereas it was lowered in the
insectary larval habitat where the bacteria inadequately
recycled. The bacteriumwas weakly transmitted transstadially
from larval to adult stage. The bacterial populations increased
smoothly and then dramatically in the guts of An. stephensi
following sugar and blood meal respectively followed by a
gradual reduction over the time.
Conclusion:
This study showed that E. cloacae was highly
stable in sugar bait and increased tremendously in the gut
of female adult An. stephensi within 24 hours post blood
meal. Sugar bait stations can be used for introduction of
the transgenic bacteria in a paratransgenic approach. It is
recommended to evaluate the attraction of sugar bait in
combination with attractive kairomones as well as its stability
and survival rate in the semi-field or field conditions.
J Parasit Dis Diagn Ther 2017