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J u l y 2 3 - 2 4 , 2 0 1 8 | R o m e , I t a l y
TRADITIONAL
MEDICINE AND ACUPUNCTURE
8
th
International Conference on
Journal of General Internal Medicine
|
ISSN: 2591-7951
|
Volume 2
Traditional 2018
EFFECT OF POMEGRANATE EXTRACTS
ON BRAIN ANTIOXIDANT MARKERS AND
CHOLINESTERASE ACTIVITY IN HIGH FAT-
HIGH FRUCTOSE DIET INDUCED OBESITY IN
RAT MODEL
Zahra Amri
1
, Asma Ghorbel
2
, Mouna Turki
3
,
Férièle Messadi Akrout
2
, Fatma Ayadi
3
, Abdelfateh Elfeki
4
and
Mohamed Hammami
1
1
Biochemistry Laboratory, LR12ES05 “Nutrition- Functional Foods and vascular
Health”, Faculty of Medicine, University of Monastir (Tunisia)
2
Laboratory of Hygiene CHU Hédi Chaker, Sfax (Tunisia)
3
Biochemistry Laboratory, CHU H. Bourguiba, Sfax (Tunisia)
4
Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax, University of
Sfax (Tunisia)
Background:
To investigate beneficial effects of pomegranate seeds oil
(PSO), leaves (PL), juice (PJ) and (PP) on brain cholinesterase activity, brain
oxidative stress and lipid profile in high-fat-high fructose diet (HFD) induced-
obese rat.
Methods:
In vitro
and
in vivo
cholinesterase activity, brain oxidative status,
body and brain weight and plasma lipid profile were measured in control rats,
HFD-fed rats and HFD-fed rats treated by PSO, PL, PJ and PP.
Results:
In vitro
study showed that PSO, PL, PP, PJ inhibited cholinesterase
activity in dose dependant manner. PL extract displayed the highest inhibitory
activity by IC50 of 151.85 mg/ml. For
in vivo
study, HFD regime induced a
significant increase of cholinesterase activity in brain by 17.4% as compared
to normal rats. However, the administration of PSO, PL, PJ and PP to HDF-
rats decreased cholinesterase activity in brain respectively by 15.48%, 6.4%,
20% and 18.7% as compared to untreated HFD-rats. Moreover, HFD regime
caused significant increase in brain stress, brain and body weight, and lipid
profile disorders in blood. Furthermore, PSO, PL, PJ and PP modulated
lipid profile in blood and prevented accumulation of lipid in brain and body
evidenced by the decrease of their weights as compared to untreated HFD-
rats. In addition, administration of these extract protected brain from stress
oxidant, evidenced by the decrease of malondialdehyde (MDA) and protein
carbonylation (PC) levels and the increase in superoxide dismutase (SOD)
and glutathione peroxidase (GPx) levels.
Conclusion:
These findings highlight the neuroprotective effects of
pomegranate extracts and one of mechanisms is the inhibition of
cholinesterase and the stimulation of antioxidant capacity.
Zahra Amri et al., Arch Gen Intern Med 2018, Volume 2 | DOI: 10.4066/2591-7951-C1-003
Zahra Amri is Doctor of Biology and a Member of Bio-
chemistry Laboratory, LR12ES05 Nutrition-Function-
al Foods and Vascular Health, Faculty of Medicine,
University of Monastir (Tunisia). She has completed
her PhD from Sfax University in April 2018, and she is
looking for a postdoctoral research position. Her PhD
subject is the investigation of the ability of bioactive
compounds extracted from fruits to ameliorate symp-
toms associated with cardiovascular diseases and
diabetes. She has published four papers in reputed
journals. She obtained a master’s degree in Molecular
and Cellular Biology in 2012, and bachelor’s degree in
2006.
zahraamri23@yahoo.frBIOGRAPHY