2 / 12
Page 48
allied
academies
J u l y 2 3 - 2 4 , 2 0 1 8 | R o m e , I t a l y
Note:
TRADITIONAL
MEDICINE AND ACUPUNCTURE
8
th
International Conference on
Journal of General Internal Medicine
|
ISSN: 2591-7951
|
Volume 2
Traditional 2018
ANTI-INFLAMMATORY ACTIVITY OF THE
DECOCTION OF
FORSYTHIA SUSPENSA
(THUNB.) VAHL IS RELATED TO THE
INDUCTION OF A20
Myungsoo Joo
and
Kyun Ha Kim
Pusan National University, Republic of Korea
T
he water extract of
Forsythiae fructus
(WFF) is a herbal remedy that has
been used to treat various inflammatory diseases in Traditional Chinese
Medicine. Although WFF has shown to suppress inflammatory reaction, the
underlying mechanisms for this activity remain less understood. Here, we
examined whether the anti-inflammatory activity of WFF is associated with
A20 or
TNFAIP3
, a ubiquitin-regulator protein that inhibits inflammatory
signaling cascades triggered by endotoxin or cytokines. The water extract
of
Forsythia suspensa
(Thunb.) Vahl was prepared and fingerprinted by
HPLC. WFF treatment of RAW 264.7 cells increased the nuclear Nrf2, an
anti-inflammatory transcription factor, and induced the expression of Nrf2-
dependent genes such as HO-1,
NQO1
, and
GCLC
, suggesting that WFF
activates Nrf2. On the other hand, WFF suppressed NF-B activity induced by
LPS or TNF-, suggesting that WFF inhibits the signaling cascades started
from the receptors for LPS and TNF-. WFF induced the expression A20,
which was coincided with the suppression of NF-κB. By contrast, when A20
expression was silenced by siRNA, WFF failed to suppress NF-κB. Therefore,
our results suggest that while activating Nrf2, WFF suppresses NF-κB by
inducing the expression of A20, which collectively attributes to the anti-
inflammatory function of WFF.
Myungsoo Joo et al., Arch Gen Intern Med 2018, Volume 2 | DOI: 10.4066/2591-7951-C1-003
Myungsoo Joo has completed his PhD from the Uni-
versity of Texas at Austin, USA. He is a Professor of
Pusan National University, Republic of Korea, publish-
ing about 70 publications in relation to lung inflamma-
tion.
mjoo@pusan.ac.krBIOGRAPHY